Protein Info for PS417_08135 in Pseudomonas simiae WCS417

Annotation: 3-phosphoshikimate 1-carboxyvinyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03807: F420_oxidored
  • amino acids 5 to 84 (80 residues), 30.5 bits, E=9e-11
• PF02153: PDH_N
  • amino acids 17 to 171 (155 residues), 127 bits, E=9.3e-41
• PF20463: PDH_C
  • amino acids 177 to 273 (97 residues), 95.2 bits, E=5.4e-31
• PF00275: EPSP_synthase
  • amino acids 307 to 720 (414 residues), 385.7 bits, E=5.1e-119
• TIGR01356: 3-phosphoshikimate 1-carboxyvinyltransferase
  • amino acids 314 to 727 (414 residues), 414.2 bits, E=2.7e-128

Best Hits

KEGG orthology group: K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 96% identity to pfs:PFLU1647)

Predicted SEED Role

"Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) or Phenylalanine and Tyrosine Branches from Chorismate (EC 1.3.1.12, EC 1.3.1.43, EC 2.5.1.19)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• superpathway of chorismate metabolism (44/59 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• L-tyrosine biosynthesis I (3/3 steps found)
• L-tyrosine biosynthesis III (3/4 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• 4-hydroxy-3-prenylbenzoate biosynthesis (1/4 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Novobiocin biosynthesis
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.19

Use Curated BLAST to search for 1.3.1.12 or 1.3.1.43 or 2.5.1.19

Sequence Analysis Tools

See A0A1N7TZF9 at UniProt or InterPro

Protein Sequence (736 amino acids)

>PS417_08135 3-phosphoshikimate 1-carboxyvinyltransferase (Pseudomonas simiae WCS417)
MIGRLVVIGLGLIGGSFAKGLRESGLCSEVVGVDLDPQSRKLAVELGVVDRCEADLALAC
QGADVIQLAVPILAMEKLLGVLAGMDLGQAILTDVGSAKGNVVRAAQRAFGGMPARFVPG
HPIAGSEQSGVEASNAQLFRRHKVILTPLEQTDPAALAVVDRLWRELGADVEHMQVERHD
EVLAATSHLPHLLAFGLVDSLAKRNENLDIFRYAAGGFRDFTRIAGSDPVMWHDIFLANR
EAVLRTLDTFRSDLDALRDAVDAGDGHQLLGVFTRARVAREHFSKILARRAYMETAVTAD
ELTFLAEPGGRLSGRIRVPGDKSISHRSIMLGSLAEGVTEVEGFLEGEDALATLQAFRDM
GVVIEGPHHGRVTIHGVGLHGLKPAPGPIYLGNSGTSMRLLSGLLAAQSFDSVLTGDASL
SKRPMSRVAKPLREMGAVIETGPEGRPPLTIRGGQSLKGLAYAMPMASAQVKSCLLLAGL
YAEGKTVVAEPAPTRDHTERMLRGFGYPVAVEGATASVESGHALAATHIEVPGDISSSAF
FLVAASIAEGSELLLEHVGVNPTRTGVIDILRLMGADITLENQREVGGEPVADLRVRAAA
LKGIEIPEALVPLAIDEFPVLFVAAACAEGRTVLRGAQELRVKESDRIQVMADGLLALGV
KCEPTADGIIIDGGLIGGGEVHAHGDHRIAMAFSVASLRAATPIRIRDCANVATSFPNFL
TLCAQVGIRVAQEAQL