Protein Info for GFF1589 in Xanthobacter sp. DMC5

Annotation: Putative acyl-CoA dehydrogenase FadE17

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 7 to 120 (114 residues), 57.9 bits, E=1.9e-19
• PF02770: Acyl-CoA_dh_M
  • amino acids 125 to 213 (89 residues), 69.9 bits, E=2.7e-23
• PF00441: Acyl-CoA_dh_1
  • amino acids 231 to 389 (159 residues), 87.4 bits, E=1.7e-28

Best Hits

KEGG orthology group: None (inferred from 95% identity to xau:Xaut_1702)

MetaCyc: 48% identical to pimeloyl-CoA dehydrogenase large subunit (Rhodopseudomonas palustris)
Pimeloyl-CoA dehydrogenase. [EC: 1.3.1.62]

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• benzoyl-CoA degradation III (anaerobic) (3/9 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Fatty acid metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2

Use Curated BLAST to search for 1.3.1.62 or 1.3.99.2

Protein Sequence (392 amino acids)

>GFF1589 Putative acyl-CoA dehydrogenase FadE17 (Xanthobacter sp. DMC5)
MDTFFSDAEQAFRAEVRAFIRANLPADLAAKVRAGIHLSRADMARWNGILDAKGWAAHHW
PQAYGGPGWTPIQRFLFEAECADADAPPLSVFGVYLVGPTLYTFGSDAQKAAYLPGIRSG
ATFWCQGYSEPNAGSDLASLKTTARKVEGGYIIDGAKAWTTEGHFADYMICLARTNPQVK
PQAGLSLFIVDMTAKGVSLQPVITIDGAHSVNTTFLDGVFVPDDALIGEIDKGWTYAKFL
LSHERTNNAQVHHSRREFTRLKELARQVNGPAGGRLLDDPVFQRRFAALDVELSALEVTV
LRVLADQTDGREPGPEASILKVIGSELQQRISELAMDVLGEEGIRQSHGEESAAATEAAG
WAERHLFRRVVTIYAGANEIQKTIIARTVLGM