Protein Info for GFF1586 in Variovorax sp. SCN45

Annotation: 2-dehydropantoate 2-reductase (EC 1.1.1.169)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 341 PF03807: F420_oxidored" amino acids 11 to 103 (93 residues), 28.1 bits, see alignment E=8.8e-10 PF00070: Pyr_redox" amino acids 11 to 44 (34 residues), 22.4 bits, see alignment 5e-08 PF03446: NAD_binding_2" amino acids 11 to 108 (98 residues), 23.4 bits, see alignment E=1.8e-08 PF02558: ApbA" amino acids 12 to 179 (168 residues), 92.8 bits, see alignment E=5.7e-30 PF13450: NAD_binding_8" amino acids 14 to 54 (41 residues), 23.7 bits, see alignment 1.7e-08 PF08546: ApbA_C" amino acids 206 to 323 (118 residues), 97.2 bits, see alignment E=3.3e-31

Best Hits

KEGG orthology group: K00077, 2-dehydropantoate 2-reductase [EC: 1.1.1.169] (inferred from 60% identity to bgd:bgla_1g26270)

Predicted SEED Role

"2-dehydropantoate 2-reductase (EC 1.1.1.169)" in subsystem Coenzyme A Biosynthesis (EC 1.1.1.169)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.169

Use Curated BLAST to search for 1.1.1.169

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (341 amino acids)

>GFF1586 2-dehydropantoate 2-reductase (EC 1.1.1.169) (Variovorax sp. SCN45)
MRTPTNGCPLRIAVIGAGAIGGHFAARLAQAGHSVTVLDRGATLEAIREHGLRYTSGPQP
QQCIAVRAAASPLEMEPPELVVLALKSHVLPAVVPTLSPLLKEGATVLPVGNGLPWWYFL
VPGKPLSGLRLRSVDPEGAIERSIDVQQVLGGSVMASCHCSEPGVVVHSAGGRVVVGEPA
GGLSERAQHWAGVLADAGLGAVASDDIRSELWLKLLGNACANPLSLLTGVTTDRMLDDDP
MRQLFERMMTECISLGRRLGLTIQTDTAQRMAQTRKLGAIKTSMLQDMEAGRGVELDAIL
GAALESASALQQPVPYLESAFALACMRAQQAGLYFSRSSAR