Protein Info for GFF1580 in Sphingobium sp. HT1-2

Annotation: Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 11 to 213 (203 residues), 95.3 bits, E=9.6e-31
• PF16113: ECH_2
  • amino acids 17 to 202 (186 residues), 67.3 bits, E=4.5e-22
• PF02737: 3HCDH_N
  • amino acids 324 to 501 (178 residues), 198.8 bits, E=1.9e-62
• PF00725: 3HCDH
  • amino acids 504 to 600 (97 residues), 70.8 bits, E=2.9e-23

Best Hits

KEGG orthology group: K01782, 3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase [EC: 1.1.1.35 4.2.1.17 5.1.2.3] (inferred from 89% identity to sch:Sphch_1081)

Predicted SEED Role

"Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Isobutyryl-CoA to Propionyl-CoA Module (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3)

MetaCyc Pathways

• oleate β-oxidation (28/35 steps found)
• adipate biosynthesis (5/5 steps found)
• adipate degradation (5/5 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
• L-isoleucine degradation I (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (12/18 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• valproate β-oxidation (5/9 steps found)
• glycerol degradation to butanol (10/16 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (17/26 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• 6-gingerol analog biosynthesis (engineered) (2/6 steps found)
• phenylacetate degradation I (aerobic) (4/9 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• 2-methylpropene degradation (3/8 steps found)
• 3-phenylpropanoate degradation (4/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (9/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• L-glutamate degradation VII (to butanoate) (5/12 steps found)
• superpathway of phenylethylamine degradation (4/11 steps found)
• methyl tert-butyl ether degradation (3/10 steps found)
• L-tryptophan degradation III (eukaryotic) (4/15 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (4/17 steps found)
• toluene degradation VI (anaerobic) (4/18 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• androstenedione degradation I (aerobic) (6/25 steps found)
• platensimycin biosynthesis (6/26 steps found)
• superpathway of testosterone and androsterone degradation (7/28 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (2/22 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (8/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (4/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.1.2.3

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3

Protein Sequence (725 amino acids)

>GFF1580 Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) (Sphingobium sp. HT1-2)
METIRFDIDADGIATLTIDVPGQSMNVIGPDFLADLDTAITRIASEEGIKGAVIASGKDS
GFMAGMDLKYFGSMLASADGQRPAPTAIFDNVFVLNQLFRRLETAGKPVACAIEGTCVGG
GFELALACHRRFVGDSPKTQLGLPEILIGLFPGGGGSQRLPRIMGVQASLMYMLQGKLFR
PAEAAMLKVVDGVVPQGTALAAAKDWVKANPTATTQPWDVKGFKVPGGAGGFNPAFVQTM
AGALPMTLKQTQRNMNAPIALLSAVYEGITLPMDRAIRIESKYFAKVAADPQASNMIRTL
FVNKQAAERGARRPKDEPKAPTTKLAMLGAGMMGAGIATVAAQAGMDVVLFDRDQAYAEK
GKAHVEAVLSKRLGKGMTPEKMAATLARVTPTTDYAALAGADFVIEAVFEDVAIKAEVTK
QVEAVLGADTIFGSNTSTLPITKLANAWFKPENFIGVHFFSPVEKMPLVEIILGEKTGPA
AIAKALDFVAQIKKTPIVVHDSRGFYTSRSFGTYVQEGAELVGEGINPALIENAGKQLGM
PTGPLAVSDEVSIELGVKIMTAAKKELGDAYVPQGSDDIMVKMVEADRLGRKNGKGWYDY
PEGGKKHLSPVLGELFPRADAQPDVEEVKERLLYRQLIECARCFEEGVLETPEDGDIGAI
FGWGFAPYTGGPFSHMDTVGIAHVVAVLDRLAAAHGPRFAPTAQLREMAANGATFYRPVP
SRAAA