Protein Info for PS417_08035 in Pseudomonas simiae WCS417

Updated annotation (from data): adenine transporter (MFS superfamily)
Rationale: specific phenotype on adenine and no other transporter was identified by the fitness data. Also mildly important on adenosine, which could reflect extracellular conversion to adenine
Original annotation: transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 transmembrane" amino acids 7 to 26 (20 residues), see Phobius details amino acids 38 to 61 (24 residues), see Phobius details amino acids 73 to 91 (19 residues), see Phobius details amino acids 97 to 119 (23 residues), see Phobius details amino acids 131 to 153 (23 residues), see Phobius details amino acids 159 to 180 (22 residues), see Phobius details amino acids 206 to 230 (25 residues), see Phobius details amino acids 243 to 260 (18 residues), see Phobius details amino acids 272 to 295 (24 residues), see Phobius details amino acids 301 to 324 (24 residues), see Phobius details amino acids 336 to 355 (20 residues), see Phobius details amino acids 361 to 382 (22 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 4 to 380 (377 residues), 334.6 bits, see alignment E=5.5e-104 PF07690: MFS_1" amino acids 12 to 343 (332 residues), 147.4 bits, see alignment E=5.4e-47 PF00083: Sugar_tr" amino acids 43 to 117 (75 residues), 36.4 bits, see alignment E=3e-13

Best Hits

KEGG orthology group: None (inferred from 91% identity to pfs:PFLU1626)

Predicted SEED Role

"Putative transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TZE6 at UniProt or InterPro

Protein Sequence (396 amino acids)

>PS417_08035 adenine transporter (MFS superfamily) (Pseudomonas simiae WCS417)
MKNSFGFTWYLAGLSMLGYLAMDMYLPAFGAMGEQLQIGAGAVGASLSIFLAGFAVGQLL
WGPLSDRLGRKPILLAGLSLFVLGCAGMFWVETAPQLLALRFIQAIGVCSAAVSWQALVI
DRYPADKAHRVFASIMPLMSLSPALAPLLGAMVLNHFGWQAIFGVLLGVSVLLLLPTVFL
RGMPKRPTEGREPVRLGYGQLLTSRVFTGNVMIFAACSASFFAWLTASPFILGGMGYSPN
DIGLSYVLPTLAFLVGGYSCRSALQRFQGKTLLPWLLLAYCISMVALYLVATLTVPTLTT
LLIPFCLMALCNGASYPIVVANALMPFSENSGKAAALQNTLQLGLCFLSSLVVSSMLDQP
LLITAIVMLATAPLAVLGYWLARPKADNSVLAPNLK