Protein Info for GFF157 in Xanthobacter sp. DMC5

Annotation: RNA pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 154 PF00293: NUDIX" amino acids 20 to 143 (124 residues), 83 bits, see alignment E=9.9e-28

Best Hits

KEGG orthology group: None (inferred from 81% identity to xau:Xaut_1476)

Predicted SEED Role

"GDP-mannose mannosyl hydrolase (EC 3.6.1.-)" in subsystem Colanic acid biosynthesis or Mannose Metabolism or Nudix proteins (nucleoside triphosphate hydrolases) (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-

Use Curated BLAST to search for 3.6.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (154 amino acids)

>GFF157 RNA pyrophosphohydrolase (Xanthobacter sp. DMC5)
MSDTHESETAVPPRVEVRPTLAASAAVFRGPLVLLARRAANPGAGLWSLPGGRVEPGETV
AEAAMRELMEEVGVEAEIVGLAAARDIIVRNREGELKAHFVVLAHAARWRGGEPMPGEEA
AEVGWFRPNEVAGLTTTEGLAEVVAKAALLMGEM