Protein Info for GFF1565 in Sphingobium sp. HT1-2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 456 transmembrane" amino acids 13 to 33 (21 residues), see Phobius details amino acids 51 to 76 (26 residues), see Phobius details amino acids 97 to 118 (22 residues), see Phobius details amino acids 138 to 155 (18 residues), see Phobius details amino acids 162 to 185 (24 residues), see Phobius details amino acids 194 to 218 (25 residues), see Phobius details amino acids 241 to 268 (28 residues), see Phobius details amino acids 276 to 299 (24 residues), see Phobius details amino acids 319 to 340 (22 residues), see Phobius details amino acids 360 to 385 (26 residues), see Phobius details amino acids 397 to 419 (23 residues), see Phobius details amino acids 425 to 447 (23 residues), see Phobius details TIGR00797: MATE efflux family protein" amino acids 20 to 424 (405 residues), 198.4 bits, see alignment E=9.1e-63 PF01554: MatE" amino acids 20 to 180 (161 residues), 87 bits, see alignment E=6e-29 amino acids 248 to 412 (165 residues), 96.8 bits, see alignment E=5.5e-32

Best Hits

Swiss-Prot: 55% identical to NORM_RHILO: Probable multidrug resistance protein NorM (norM) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K03327, multidrug resistance protein, MATE family (inferred from 80% identity to sch:Sphch_0423)

Predicted SEED Role

"Multidrug and toxin extrusion (MATE) family efflux pump YdhE/NorM, homolog"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (456 amino acids)

>GFF1565 hypothetical protein (Sphingobium sp. HT1-2)
VTQSSPWRIEARALVTLAIPMIAGNVAWSGIAATDLLLVGRLGAEAVAAGALAINLFNAL
LIFGLGLVTAAAPLIASERGRRRHSVRDVRRTVHQTLRAAAFFVLPAWLILWHCEAILRA
MGQEPDLAREAGHLMRGMQWALLPFLCFTTLRNFISALERPLWGLIIMLLAIPFNVLVGW
ALIFGHLGLPALGLLGAGIASTLSSTFLFLGLLTVILVDRRFRRYRLMGRFWTRDRDRQR
AVWTLGLPIAITLGLEVTVFNASAFLMGLIDRESLAAHAVAIQIASLVFMVPMGIGQAAT
VRVGVAYGRADPAAIGRAGWLALIVGTGFAMAAALMLVLIPRPLVSIFLDVHDPANLATA
NLAVSFLAVAALFQLVDAAQAVGAGVLRGIQDTKVPMIFALFGYWIIGIGVGALLAFPMG
LHGVGIWFGLAAGLGTVAILLIARWSLRDRLGLVLR