Protein Info for PS417_07955 in Pseudomonas simiae WCS417
Annotation: malate:quinone oxidoreductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 85% identical to MQO2_PSEPK: Probable malate:quinone oxidoreductase 2 (mqo2) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 98% identity to pfs:PFLU1613)MetaCyc: 67% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]
Predicted SEED Role
"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
- superpathway of glyoxylate bypass and TCA (11/12 steps found)
- partial TCA cycle (obligate autotrophs) (8/8 steps found)
- TCA cycle I (prokaryotic) (9/10 steps found)
- TCA cycle VI (Helicobacter) (7/9 steps found)
- TCA cycle VII (acetate-producers) (7/9 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.5.4
Use Curated BLAST to search for 1.1.5.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7U900 at UniProt or InterPro
Protein Sequence (548 amino acids)
>PS417_07955 malate:quinone oxidoreductase (Pseudomonas simiae WCS417) MFKKVNTALLGLALSLGISSVHAEEAKKVDVLLIGGGIMSATLGVWLNELEPGWSMEMVE RLDGVAEESSNGWNNAGTGHSALAELNYTPEDKNGNVEIPKAVEINEAFQISRQFWAWQV RQGVLKDPRSFINTTPHMSFVWGDDNIKFLKKRYEALKASPLFAGMQYSEDPAQIAKWVP LMMEGRDPNQKIAATWSPLGTDMNFGEITRQFVAHLQTTPKFDLKLSSEVQDITKNADGS WRVSYKNLKDGTKTETDAKFVFIGAGGGALHLLQKSGIPEAKEYAGFPVGGSFLVTDNPT VAEQHLAKAYGKASVGAPPMSVPHLDTRVLDGKRVILFGPFATFSTKFLKEGSYLDLLTS TTTHNIWPMTKVGIREYPLVEYLAGQLMLSDDDRFKALQEYFPNAKQSDWRLWQAGQRVQ IIKRDEEQGGVLKLGTEVVSSADNTIAGLLGASPGASTAAPIMLTVLQKVFKDKVATPEW QTKLHQIVPSYGTKLNDSPEAVAKEWAYTAEILQLPTPPVIPQVAAPKATEAAKPAAEPS KPASDLAL