Protein Info for GFF1550 in Sphingobium sp. HT1-2

Annotation: ABC transporter, RND-adapter-like protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 transmembrane" amino acids 26 to 45 (20 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 59 to 356 (298 residues), 132.4 bits, see alignment E=9e-43 PF16576: HlyD_D23" amino acids 79 to 286 (208 residues), 65.1 bits, see alignment E=8.4e-22 PF13533: Biotin_lipoyl_2" amino acids 80 to 128 (49 residues), 40.4 bits, see alignment 3.1e-14 PF13437: HlyD_3" amino acids 208 to 331 (124 residues), 51.6 bits, see alignment E=2.2e-17

Best Hits

KEGG orthology group: K02005, HlyD family secretion protein (inferred from 80% identity to sjp:SJA_C1-16020)

Predicted SEED Role

"Macrolide-specific efflux protein MacA" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (515 amino acids)

>GFF1550 ABC transporter, RND-adapter-like protein (Sphingobium sp. HT1-2)
MSNDASPDQDLNDFLGAKPEKPHRKWLIRGAIGVVLLILVLLVARCFAGDDKPNYATREA
RQGDLTVSVSATGNLKPINQVDVGSEQSGKITAVYVDVNDRVTKGQKLAELDTRRLVDTI
NQTQAQVASSQAGVAQAQAQVALAKATLDRQLNVYQLSGGKVPAKTELDSARADYNGALA
NLRSAQAQVNVSRAQLSTAQTNLSIAQIVSPVTGVVLSRDIEPGQTVAASLNAPVLFTIA
EDLTKMEVEVSVDEADVGQVKEGQTANFAVDAFPGRTFPAKVTRVNVGSNSSSSSSSSSS
STSTTSSSTGTVVAYTAVLSVDNADETLRPGMTATADIVTQELHDVLLVPNSALRFKPSS
GAGSGGGITSVLPGPGRMRRSGANRQVNFGAGSSQTLYVLDEKGDPKAIQVTVGASDGSR
TVITGGDLKPGMKVITGQLAAGAQQPAEDQKADHAAGDTKDRPRNPAADGSPASVKSLGN
SGDAAPQTAPVAPTPALPQGSGGAESRGTGRQSGT