Protein Info for GFF1544 in Variovorax sp. SCN45

Annotation: Two-component system sensor histidine kinase/response regulator hybrid

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 662 PF00072: Response_reg" amino acids 18 to 129 (112 residues), 62.1 bits, see alignment E=1.6e-20 amino acids 541 to 655 (115 residues), 65.7 bits, see alignment E=1.2e-21 TIGR00229: PAS domain S-box protein" amino acids 143 to 263 (121 residues), 56.1 bits, see alignment E=2.1e-19 PF13188: PAS_8" amino acids 147 to 192 (46 residues), 29 bits, see alignment 2.2e-10 PF00989: PAS" amino acids 148 to 244 (97 residues), 34.5 bits, see alignment E=5.4e-12 PF00512: HisKA" amino acids 285 to 350 (66 residues), 55 bits, see alignment E=2e-18 PF02518: HATPase_c" amino acids 397 to 505 (109 residues), 90 bits, see alignment E=4.4e-29

Best Hits

Predicted SEED Role

"Histidine kinase/response regulator hybrid protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (662 amino acids)

>GFF1544 Two-component system sensor histidine kinase/response regulator hybrid (Variovorax sp. SCN45)
MPPEPLINTTIDRSRHTVLVVDDNPTTRYATARTIRAAGFQTREAGTGGEALALASQHIS
AVVLDVHLPDFTGFEVCHELRIKPSTATLPVVHLSATFVRNEDKVAGLNAGADAYLVHPV
EPPVLIATLQALIRARMAEEKQRNSEARFRAIYDHAPTGIALIDAQGCFADVNPALVAML
GQSREVLVGQPVSAFAPDEWTAFVKTHTQGVQPPSAPWTGEFPLLRLDGSWIRLEWTMSG
HIEPGLRVGIALDVSERFELEARRREVLEREQAARVAAERLSRTKDDFVAVLSHELRNPL
NVIGGWVHVLKAGGRTPEQMEKGLTAIDRSVKAQTRLIADILDVSRISSGKLRLHREWAE
PRSLVSAAIEALMAPVAAKRLNILFDRQEADPPAWLDATRFQQIVWNLLSNAIKFSDEGG
RILIQLQRQDHMLHLSVQDFGRGIRADFLHHLFDRFSQSDSPDNRLHGGLGLGLSIVKNL
AELHGGSVSAHSEGEGLGATLRVTLGVIPSDDTPLLLVDALDSRAGGAAAVTDRTLHGLD
VLLVEDNADASEIITVVLADAGATVRLALDVEGALQLFDQKWPDVLISDIGLPGRDGYDL
IREVRQRERTLGKPRLFSIAHTAFTRPQDLAKTTEAGFDAHLGKPLQPHALVALVAGHAA
PG