Protein Info for GFF1541 in Sphingobium sp. HT1-2

Annotation: Homoserine O-succinyltransferase (EC 2.3.1.46)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01001: homoserine O-succinyltransferase
  • amino acids 1 to 296 (296 residues), 333.6 bits, E=7e-104
• PF04204: HTS
  • amino acids 2 to 294 (293 residues), 411.2 bits, E=1.1e-127

Best Hits

Swiss-Prot: 75% identical to METAA_SPHWW: Homoserine O-acetyltransferase (metAA) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K00651, homoserine O-succinyltransferase [EC: 2.3.1.46] (inferred from 80% identity to sjp:SJA_C1-26590)

MetaCyc: 59% identical to homoserine O-acetyltransferase (Agrobacterium fabrum C58)
Homoserine O-acetyltransferase. [EC: 2.3.1.31]

Predicted SEED Role

"Homoserine O-succinyltransferase (EC 2.3.1.46)" in subsystem Methionine Biosynthesis (EC 2.3.1.46)

MetaCyc Pathways

• aspartate superpathway (25/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• L-methionine biosynthesis I (5/5 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.31

Use Curated BLAST to search for 2.3.1.31 or 2.3.1.46

Protein Sequence (308 amino acids)

>GFF1541 Homoserine O-succinyltransferase (EC 2.3.1.46) (Sphingobium sp. HT1-2)
VPIKIADDLPARRTLEAEGVAVMGEADAVRQDIRPLRIALLNLMPNKIDTETQLARLLGA
SPLQVELTLVRISDHVSRNTSADHMQSFYRAWTDVRDERFDGFLITGAPVEHLPFEEVSY
WQELRAIFDWTQTHVHRSLSICWAAQAALHHFHGVEKHALERKAFGVFRHRNHAPASPYL
RGFSDDFSIPVSRWSEVRRESLPVDRGLQLLADSAETGPCLVHDPRHGFLHMFNHVEYDT
RTLGDEYARDGGRQMPAHYFPDEDPTRPPENRWRSHAHLLFGNWINDLYQTAPFDVDMIG
QEILQSAA