Protein Info for GFF1530 in Variovorax sp. SCN45

Annotation: Nucleoside-diphosphate-sugar epimerases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF05368: NmrA" amino acids 2 to 70 (69 residues), 38.6 bits, see alignment E=1.5e-13 PF01370: Epimerase" amino acids 3 to 211 (209 residues), 48.1 bits, see alignment E=1.6e-16 PF13460: NAD_binding_10" amino acids 7 to 115 (109 residues), 39 bits, see alignment E=1.2e-13

Best Hits

Swiss-Prot: 46% identical to YGI2_SCHPO: Uncharacterized protein C2A9.02 (SPBC2A9.02) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: None (inferred from 63% identity to fre:Franean1_3653)

Predicted SEED Role

"NAD-dependent epimerase/dehydratase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (297 amino acids)

>GFF1530 Nucleoside-diphosphate-sugar epimerases (Variovorax sp. SCN45)
MRILVTGASGWIGSASVVELISAGHHVLGLARNEEAEAKIRRLGAEVVPGSLDDLASLRA
AAARADGVVHLGYKHDFSQMADAARTDRAAIDAFADVLQGTGGPLLIASGTLGLAPGRVG
TESDMPNPAVHPRVGNAAHALGLAERGIRSLVVRFAPTVHGAGGDHGFVATLAKIARAKG
VSAYLGEGRNRWSAVNRLDAARLVRLAIDKAQPGSVLHAVAEEAIAARDIASALGQALEL
PVASIPVDRAAEHFDWIGMFFGADAPASNARTRELLGWEPTHATLLEDIAAGHYPGS