Protein Info for GFF153 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase (EC 6.3.2.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 transmembrane" amino acids 281 to 303 (23 residues), see Phobius details TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 1 to 467 (467 residues), 541.2 bits, see alignment E=1.2e-166 PF01225: Mur_ligase" amino acids 2 to 78 (77 residues), 38.7 bits, see alignment E=1.6e-13 PF08245: Mur_ligase_M" amino acids 90 to 294 (205 residues), 180.5 bits, see alignment E=5.7e-57 PF02875: Mur_ligase_C" amino acids 315 to 401 (87 residues), 101.5 bits, see alignment E=3.8e-33

Best Hits

Swiss-Prot: 100% identical to MURE_SALTY: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 99% identity to sec:SC0120)

MetaCyc: 92% identical to UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase. [EC: 6.3.2.13]

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (471 amino acids)

>GFF153 UDP-N-acetylmuramoylalanyl-D-glutamate--2,6- diaminopimelate ligase (EC 6.3.2.13) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MTLDSRVAAAGDLFVAVVGHQADGRRYIPQAIAQGVAAIIAEAKDEASDGEIREMHGVPV
VYLSQLNERLSALAGRFYHEPSENMRLVAVTGTNGKTTTTQLLAQWSQLLGETSAVMGTV
GNGLLGKVIPTENTTGSAVDVQHVLASLVAQGATFGAMEVSSHGLVQHRVAALKFAASVF
TNLSRDHLDYHGDMAHYEAAKWMLYSTHHHGQAIVNADDEVGRRWLASLPDAVAVSMEGH
INPNCHGRWLKAEAVEYHDRGATIRFASSWGEGEIESRLMGAFNVSNLLLALATLLALGY
PLTDLLKTAARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVF
GCGGDRDKGKRPLMGAIAEEFADIVVVTDDNPRTEEPRAIINDILAGMLDAGQVRVMEGR
AEAVTNAIMQAKDNDVVLIAGKGHEDYQIVGTQRLDYSDRVTAARLLGVIA