Protein Info for GFF1528 in Variovorax sp. SCN45

Annotation: Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 417 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 160 to 181 (22 residues), see Phobius details amino acids 214 to 233 (20 residues), see Phobius details amino acids 368 to 389 (22 residues), see Phobius details PF03929: PepSY_TM" amino acids 7 to 391 (385 residues), 202.8 bits, see alignment E=5.4e-64

Best Hits

KEGG orthology group: None (inferred from 62% identity to pmk:MDS_4882)

Predicted SEED Role

"Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (417 amino acids)

>GFF1528 Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB (Variovorax sp. SCN45)
MRAWMTRLHRWAGLFMAAFLFVAGLTGAVISWDHEIDEWLNPRLFDAPAPSPEARKRTAL
ELIEGLEAADPRLRVAFAPLEVEDGHALGVSVVPRIDPATGKPHELGFNQVTLDPYTGQV
QGTRLWGAISLSRENLMPFLYKLHYSMHIPNVGALELGTWFMGMVSIVWVLDCIVALYIS
LPGARAWRRSWRKSFGFRLRQGGHKRTFDLHRAGGLWVWGLLLIVAITSVSMNLRREVVR
PLVERLSTLTPDVFALRAPVPMARFVEPGIRREEALSLARQEADRRGWQTPAGAVFYSAP
YRLYGVNFFEPGEDHASWGLGNPWVYLDAQTGAYLGDRVPGTGTMGDLFLQAQLPLHTGR
IAGTTGRILMTALGLVVAMLSVTGVMIWARKRRAALRAGHSGGASQGALAMRPPRPY