Protein Info for GFF1526 in Sphingobium sp. HT1-2

Annotation: Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.5.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 809 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 44 to 62 (19 residues), see Phobius details amino acids 69 to 87 (19 residues), see Phobius details amino acids 90 to 91 (2 residues), see Phobius details amino acids 93 to 114 (22 residues), see Phobius details amino acids 126 to 148 (23 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 18 to 808 (791 residues), 1058.7 bits, see alignment E=0 PF01011: PQQ" amino acids 229 to 254 (26 residues), 26 bits, see alignment (E = 5.8e-10) amino acids 452 to 482 (31 residues), 26.6 bits, see alignment (E = 3.6e-10) amino acids 738 to 773 (36 residues), 22.4 bits, see alignment (E = 7.9e-09) PF13360: PQQ_2" amino acids 686 to 779 (94 residues), 22.4 bits, see alignment E=8.7e-09

Best Hits

Swiss-Prot: 61% identical to QUIA_ACIAD: Quinate/shikimate dehydrogenase (quinone) (quiA) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K05358, quinate dehydrogenase (quinone) [EC: 1.1.5.8] (inferred from 78% identity to atu:Atu3354)

MetaCyc: 61% identical to quinate/shikimate dehydrogenase (quinone) (Acinetobacter baylyi ADP1)
1.1.99.25-RXN [EC: 1.1.5.8]; 1.1.5.8 [EC: 1.1.5.8]

Predicted SEED Role

"Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25)" in subsystem Chorismate Synthesis or Quinate degradation (EC 1.1.99.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.8, 1.1.99.25

Use Curated BLAST to search for 1.1.5.8 or 1.1.99.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (809 amino acids)

>GFF1526 Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.5.8) (Sphingobium sp. HT1-2)
VSGTKMHGQPLVRGWLLLLGAVILAAGLFFAIGGGKLVSLGGSPYFLIAGIALVLAGGLI
ALRKTSGGLLFGATFLLTVLWALWEVGFSFWPLISRLLAMGVGATVVALSYPLLRRGDGS
APAWRPALLMATLVGLGSAAGAAGMFMVHPTVPFAGQVASLVPVDPAREQKDWTAYGNTP
GGSRFVALDQITRDNVNELKVAWTYRTGDVAQSDGNGAEDQNTPLQVGDTLFICTPHNNV
IALDADTGREKWKTLINAKASVWMRCRGLAYFDVHAPIQQPSLPGATPVTAATVPTGAAC
QRRILMNTINAELIALDANTGKYCADFGTNGRVDLKAGLGDAPDPQYQLTSAPTLAGTTV
VVGGRVADNVQVDMPGGVMRGFDVITGKLRWAFDPGNPAITGAPPAGKTYARSTPNVWAA
MSYDPASNTVFMPVGSASVDLYGVTRSALDHTYGASMLALDATTGREKWHFQTVHNDLWD
FDLPMQPSFVDFPVDAARKVPALVFGTKAGQVYVLDRATGKPLTDVKEVPVKPANIPDEP
YSPTQPRSLGMPQIGVGPLTEADMWGATPFDQLLCRIAFKGMRYEGLYTAPGTDKSLSFP
GSLGGMNWGGISTDPTSGTIFVNDMRLGLWVQMFPQTAAQKGQSGSAGEAVNTGMGSVPL
KGTPYSVVKDRFLSALGIPCQAPPYGTLTAIDLKSRAIKWQVPVGTVRDTGPMGIRMGLP
IPIGMPTLGGTLATQGGLVFIAGTQDFYLRAFNAATGAEVWKARLPVGSQGGPMSYKSPR
SGKQYVVVTAGGARQSPDRGDYVIAYTLR