Protein Info for GFF1523 in Sphingobium sp. HT1-2

Annotation: NADH dehydrogenase (EC 1.6.99.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07992: Pyr_redox_2
  • amino acids 8 to 343 (336 residues), 164.4 bits, E=1.4e-51
• PF00070: Pyr_redox
  • amino acids 176 to 260 (85 residues), 31.6 bits, E=7.1e-11

Best Hits

Swiss-Prot: 52% identical to DHNA_ECOLI: NADH dehydrogenase (ndh) from Escherichia coli (strain K12)

KEGG orthology group: K03885, NADH dehydrogenase [EC: 1.6.99.3] (inferred from 53% identity to zmo:ZMO1113)

MetaCyc: 52% identical to NADH:quinone oxidoreductase II (Escherichia coli K-12 substr. MG1655)
RXN0-5330 [EC: 1.6.5.9]; 1.6.5.9 [EC: 1.6.5.9]; 1.16.1.- [EC: 1.6.5.9]

Predicted SEED Role

"NADH dehydrogenase (EC 1.6.99.3)" in subsystem Carboxysome or Respiratory dehydrogenases 1 (EC 1.6.99.3)

MetaCyc Pathways

• superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (7/8 steps found)
• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NADH to cytochrome bd oxidase electron transfer II (2/2 steps found)
• mitochondrial NADPH production (yeast) (4/5 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• aerobic respiration II (cytochrome c) (yeast) (3/4 steps found)
• NADH to cytochrome aa₃ oxidase electron transfer (1/2 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NADH to cytochrome bo oxidase electron transfer II (1/2 steps found)
• NADH to nitrate electron transfer (1/2 steps found)
• nitrate reduction VIIIb (dissimilatory) (1/2 steps found)
• NAD(P)/NADPH interconversion (3/6 steps found)
• Fe(II) oxidation (2/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.6.5.9 or 1.6.99.3

Protein Sequence (439 amino acids)

>GFF1523 NADH dehydrogenase (EC 1.6.99.3) (Sphingobium sp. HT1-2)
LIPDKKTQIVIVGGGAAGLELARKLGARYGRKRHDIILIDRNRTHIWKPLLHEVATGSLD
ASLDEVGYRSHCHRWGYRYLYGTLQGIDRTARRVHIAPVFDPKGREVVSAHSVRYDYLVL
AYGSVTNDFGTPGVADNCLFLDSRAQADSFRDQLLDHCLRVSRAMSADLASDARVRIAIV
GGGATGVELAAELYNAADALGYYGLEVFDRQRLDVTLLEAGPRILPALPDRLADAAREEL
EVLGVKVRAGVAVTASTPEGMETKDGGFVPADLQVWAAGVKAAAIRDGLDGLELSRAGQV
IVRPTLQSLADDRVFAMGDCASYTPQGADRPIPPRAQAAHQMADTLFANLGRLMADRPLR
TFVYKDHGSLVSLSRYSTVGSLMGKLVGGRMAVEGRLARFVYMSLYRMHLLAVHGNIRGP
ALILVSHINRFVRPRLKLH