Protein Info for GFF1500 in Sphingobium sp. HT1-2

Annotation: Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4) AsnH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03108: exosortase A system-associated amidotransferase 1
  • amino acids 1 to 626 (626 residues), 930.2 bits, E=6.8e-284
• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 561 (560 residues), 497.3 bits, E=6.4e-153
• PF13522: GATase_6
  • amino acids 33 to 162 (130 residues), 150.7 bits, E=3.6e-48
• PF13537: GATase_7
  • amino acids 48 to 168 (121 residues), 161.5 bits, E=1.3e-51
• PF00733: Asn_synthase
  • amino acids 245 to 622 (378 residues), 333.1 bits, E=4.7e-103

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 92% identity to sch:Sphch_1229)

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4) AsnH" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.4

Use Curated BLAST to search for 6.3.5.4

Protein Sequence (631 amino acids)

>GFF1500 Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4) AsnH (Sphingobium sp. HT1-2)
MCGIAGIFHLETAKPVDPARVRAMLDVMPHRGPDGAGIWTAPGVGLGHLRLSIIDLGGGA
QPMLTEDESLVVTFNGEIYNFAEVRAELEAKGHVFRTDSDTEVILHGYRQWGEECVSRFN
GMFAFALFDARAQSLWLVRDRLGVKPLHYALLSDGSLIFGSELKSLLAHPLLRRAPDLSA
VEDYLAYGYVPDDACMVAGVRKLGAGETLRLVRGRPLPHPQRYWDISFADRSKAKPEALE
EELVALMRQAVRSRMVSDVPLGAFLSGGVDSSSVVALMAEASNQAVKTCTIGFDVASLDE
TAYADRIARRFATDHRTRIVSPDDYGLADTLAFHFDEPFADASALPTYRVCELAREQVTV
ALSGDGADEAFAGYRRHRFQMQGERLRGLIPASVREPLFGTLGRYYPKADWAPRLLRAKS
TFLELAGDGGEAYAASVGVTPHALRQRLFSQDMKSRLGAYRAEDRYIKAMANAPARDPLD
RAQYADIRIWLPGDILTKSDRMSMAVSLEAREPLLDHRLVEFAARLPVAQRIRGNSGKYL
MKKAMEPFLPQDILYRPKMGFVTPISAWFRGALAGEATAVAGGSALARTGWFDTKMLAKI
AADHKAGISDHGRLLWQMVMLDKALGRLFGA