Protein Info for GFF150 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21799: MurD-like_N
  • amino acids 7 to 94 (88 residues), 126.8 bits, E=4.9e-41
• TIGR01087: UDP-N-acetylmuramoylalanine--D-glutamate ligase
  • amino acids 8 to 435 (428 residues), 428.2 bits, E=2.2e-132
• PF08245: Mur_ligase_M
  • amino acids 110 to 280 (171 residues), 88.4 bits, E=9.6e-29
• PF02875: Mur_ligase_C
  • amino acids 301 to 370 (70 residues), 40.2 bits, E=5.2e-14

Best Hits

Swiss-Prot: 100% identical to MURD_SALTY: UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 100% identity to sty:STY0146)

MetaCyc: 92% identical to UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase. [EC: 6.3.2.9]

Predicted SEED Role

"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.9

Protein Sequence (438 amino acids)

>GFF150 UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MADYQDKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRVTPPGLDKLPQEVERHVGGLND
EWLLAADLIVASPGIALAHPSLSAAASAGVEIVGDIELFCREAQAPIVAITGSNGKSTVT
TLVGEMAKAAGVNVGVGGNIGLPALMLLDADRELYVLELSSFQLETTSSLQAAAATVLNV
TEDHMDRYPFGLQQYRAAKLRVYEKAKVCVVNADDALTMPVRGADERCVSFGVNMGDYHL
NRQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAALALADAVGLPRASSLKALTTFTGL
AHRFQLALEHNGVRWINDSKATNVGSTEAALNGLHVDGTLHLLLGGDGKSADFSPLTRYL
TGDRIRLYCFGRDGAQLAALRPEIAQQTETMEEAMRLLAPRVQPGDMVLLSPACASLDQF
KNFEQRGDVFTRLAKELG