Protein Info for GFF149 in Variovorax sp. SCN45

Annotation: Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form (EC 2.3.1.164)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03417: AAT
  • amino acids 142 to 371 (230 residues), 56.9 bits, E=1.3e-19

Best Hits

KEGG orthology group: K10852, isopenicillin-N N-acyltransferase [EC: 2.3.1.164] (inferred from 89% identity to vap:Vapar_5952)

Predicted SEED Role

"Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form (EC 2.3.1.164)" (EC 2.3.1.164)

MetaCyc Pathways

• penicillin K biosynthesis (1/1 steps found)
• penicillin G and penicillin V biosynthesis (3/4 steps found)
• superpathway of penicillin, cephalosporin and cephamycin biosynthesis (1/10 steps found)

KEGG Metabolic Maps

• Penicillin and cephalosporin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.164

Use Curated BLAST to search for 2.3.1.164

Protein Sequence (386 amino acids)

>GFF149 Acyl-coenzyme A:6-aminopenicillanic-acid-acyltransferase 40 kDa form (EC 2.3.1.164) (Variovorax sp. SCN45)
MTTPQYFPLVDVSGTPHARGVQHGRAVPERVAGGVALYRAQLGRRGLDDATIARLARSML
PLIEAYDASYLEEMRGIAEGAGVPLEDIVMINCRTEMLFGYADMKSSAEPKAKVDTDAAS
SEDGDCTGLVVLPARSATGRLMHAHNWDWRQECVDTGIVLRIRTEGGPDMLTFTEAGALA
RHGFNSNGVSLTGNSLTCHEDFQQGAGVPLVLLRRRVLEAANLAQAMKTIWASKRYCANN
MILAQATGNDGSGISLETSPGEIFWTLPENDLLVHANHWMSPSAIVKLRDPGLPQRPDSI
YRQHRVENALRRVTAQPEGRRIDWSHVKAALADEFGKPDGVLRTPKPADFSSISATVATT
LMDAANRVMWVARKPYEARNFQEYTL