Protein Info for PS417_07535 in Pseudomonas simiae WCS417

Annotation: erythronate-4-phosphate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 380 PF00389: 2-Hacid_dh" amino acids 21 to 279 (259 residues), 62.4 bits, see alignment E=5.6e-21 PF02826: 2-Hacid_dh_C" amino acids 108 to 255 (148 residues), 118 bits, see alignment E=4.8e-38 PF11890: DUF3410" amino acids 288 to 376 (89 residues), 87.3 bits, see alignment E=7.5e-29

Best Hits

Swiss-Prot: 93% identical to PDXB_PSEFS: Erythronate-4-phosphate dehydrogenase (pdxB) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K03473, erythronate-4-phosphate dehydrogenase [EC: 1.1.1.290] (inferred from 93% identity to pfs:PFLU1546)

MetaCyc: 48% identical to erythronate-4-phosphate dehydrogenase (Escherichia coli K-12 substr. MG1655)
4-phosphoerythronate dehydrogenase. [EC: 1.1.1.290]

Predicted SEED Role

"Erythronate-4-phosphate dehydrogenase (EC 1.1.1.290)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 1.1.1.290)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.290

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TVT2 at UniProt or InterPro

Protein Sequence (380 amino acids)

>PS417_07535 erythronate-4-phosphate dehydrogenase (Pseudomonas simiae WCS417)
MLIVADENIPLLDAFFQGFGEIRRVPGRSIDRATVEQADVLLVRSVTNVNRALLEGTKVR
FVGTCTIGTDHLDLDYFKQAGIQWSSAPGCNARGVVDYVLGSLMTLAEIEGADLNHRTYG
VVGAGEVGGRLIKVLKGLGWNVLVCDPPRQIAEDGDYVSLDQIIEQCDVISLHTPLTKSG
NGSTWHLFDRQRLNQLKPGTWLINASRGPVVDNAALREVLLAREDLQAVLDVWEGEPEVD
VDLADLCVLATPHIAGYSLDGKQRGTAQIYQAFCTHLGQEPGIQLSDLLPQPWLAEVQLN
ASTDPAWALATLCRSVYDPRRDDADFRRSLVGTVEEQRKAFDLLRKNYPERREIDGLRVS
INGESAALSNIITALGAAGV