Protein Info for PS417_07530 in Pseudomonas simiae WCS417

Annotation: multidrug transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details amino acids 61 to 80 (20 residues), see Phobius details amino acids 102 to 124 (23 residues), see Phobius details amino acids 138 to 156 (19 residues), see Phobius details amino acids 169 to 190 (22 residues), see Phobius details amino acids 198 to 221 (24 residues), see Phobius details amino acids 249 to 273 (25 residues), see Phobius details amino acids 285 to 308 (24 residues), see Phobius details amino acids 322 to 345 (24 residues), see Phobius details amino acids 358 to 375 (18 residues), see Phobius details amino acids 396 to 417 (22 residues), see Phobius details amino acids 429 to 449 (21 residues), see Phobius details TIGR00797: MATE efflux family protein" amino acids 27 to 423 (397 residues), 365.3 bits, see alignment E=1.8e-113 PF01554: MatE" amino acids 27 to 186 (160 residues), 136.3 bits, see alignment E=4.1e-44 amino acids 253 to 413 (161 residues), 124.3 bits, see alignment E=1.9e-40

Best Hits

Swiss-Prot: 69% identical to PMPM_PSEAE: Multidrug resistance protein PmpM (pmpM) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03327, multidrug resistance protein, MATE family (inferred from 97% identity to pfs:PFLU1545)

Predicted SEED Role

"Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UHU6 at UniProt or InterPro

Protein Sequence (464 amino acids)

>PS417_07530 multidrug transporter (Pseudomonas simiae WCS417)
MNTATAPLTRPARISREVRGLLTLALPIMIGQLATTAMSFVDAVMAGRVSPQDLAAVGLG
NSIWIPVYLLMTGTLLATTPKVAQRYGAGQFSEIGPLVRQSLWLAVVVGITASLLLLCAE
PILHVMNVEPDLIEPSMGYLHGIAAGMPAIALYYVLRCFSDGLGRTRPSMVMGLCGLALN
IPLNYIFIYGHLGVPAMGGVGCGWATAIAMWVMMLGLAVWTRWGPAYQSSELYKRFDWPQ
WAVIKRVLSIGLPIGIAIFAESSIFAVIALLIGSLGATVVSGHQIALNVSSLVFMIPYSL
SMAVTVRVGQALGRGEPREARFAAGVGMGTALAYACLSCSLMLLFREQIAAIYTPDPVVI
HLASMLIVFSALFQFSDSIQVTAAGALRGYQDTRVTMVLTLFAYWGVGLPVGYALGLTNW
LGEPSGPSGLWQGLIVGLSCAAGMLLVRLARSARRRIRADKARG