Protein Info for GFF147 in Sphingobium sp. HT1-2

Annotation: ATP-dependent hsl protease ATP-binding subunit HslU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 TIGR00390: ATP-dependent protease HslVU, ATPase subunit" amino acids 5 to 433 (429 residues), 569.5 bits, see alignment E=2.6e-175 PF07728: AAA_5" amino acids 53 to 89 (37 residues), 23.6 bits, see alignment 1.1e-08 PF00004: AAA" amino acids 54 to 108 (55 residues), 27.6 bits, see alignment 9.3e-10 amino acids 234 to 322 (89 residues), 27.2 bits, see alignment E=1.2e-09 PF07724: AAA_2" amino acids 190 to 319 (130 residues), 93.4 bits, see alignment E=4.1e-30

Best Hits

Swiss-Prot: 65% identical to HSLU_ROSDO: ATP-dependent protease ATPase subunit HslU (hslU) from Roseobacter denitrificans (strain ATCC 33942 / OCh 114)

KEGG orthology group: K03667, ATP-dependent HslUV protease ATP-binding subunit HslU (inferred from 94% identity to sch:Sphch_0752)

Predicted SEED Role

"ATP-dependent hsl protease ATP-binding subunit HslU" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (433 amino acids)

>GFF147 ATP-dependent hsl protease ATP-binding subunit HslU (Sphingobium sp. HT1-2)
MNDNLTPKAIVAALDAHIIGQHDAKRAVAVALRNRWRRQHLPADLRDEVTPKNILMIGPT
GCGKTEISRRLAKLADAPFVKVEATKFTEVGYVGRDVEQIVRDLVEEAVRLEKDRRREAV
REAASEAAMARLLDALTGKDASEATRLSFRQKIEGNQMNDVEVEVEVADAPSMPMEIPGM
GGQVGMINLSDMMSKAFGQQQKKRRKMRVADAWDKLVEEEQDKRLDQDDVARVAITSAEQ
NGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLPLIEGTTVSTKYGPLKTDHILFIASGAF
HVAKPSDLLPELQGRLPIRVELKALTEEDFVAILSDTKASLVAQYRALLATEEVTIDLTP
EGIRAVAKIAAEVNSEVENIGARRLQTVMEKLLEDVSFEAEDRRGETLVIDQAYVDKQLS
AVAHDTDLSKYVL