Protein Info for GFF1463 in Sphingobium sp. HT1-2

Annotation: Serine/threonine protein phosphatase (EC 3.1.3.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K07313, serine/threonine protein phosphatase 1 [EC: 3.1.3.16] (inferred from 63% identity to sjp:SJA_C1-15750)

Predicted SEED Role

"Protein serine/threonine phosphatase PrpC, regulation of stationary phase" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase

KEGG Metabolic Maps

T cell receptor signaling pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (258 amino acids)

>GFF1463 Serine/threonine protein phosphatase (EC 3.1.3.16) (Sphingobium sp. HT1-2)
MAHPMLKFLRRSDNQDGTPPSVGDDWRVYAIGDIHGRLDLLDQLLGMLVADDALRPGKRR
CLILLGDLIDRGPHSAQVVERVRALHGSGSDIRLLKGNHEEIFVMAARGDQGAVGFFRRI
GGMETLASYGLPLSLSADMDDGAIAHWMMNYIPRADVDFLDSFVDHLTVGDYLFVHAGIR
PRVPIAEQRPSDLRWIRGDFLNHGGRHDKMVVHGHSITPKVENLANRIGIDTGAYYSGRL
TAIGLEGADRWFLQTGET