Protein Info for GFF146 in Sphingobium sp. HT1-2

Annotation: Protease II (EC 3.4.21.83)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 708 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF02897: Peptidase_S9_N" amino acids 30 to 431 (402 residues), 346.5 bits, see alignment E=2.9e-107 PF02129: Peptidase_S15" amino acids 487 to 591 (105 residues), 24 bits, see alignment E=4.4e-09 PF00326: Peptidase_S9" amino acids 491 to 705 (215 residues), 209.6 bits, see alignment E=6.3e-66

Best Hits

Swiss-Prot: 62% identical to DAPB1_PSEMX: Dipeptidyl aminopeptidase BI (dapb1) from Pseudoxanthomonas mexicana

KEGG orthology group: K01354, oligopeptidase B [EC: 3.4.21.83] (inferred from 67% identity to pzu:PHZ_c1071)

Predicted SEED Role

"Protease II (EC 3.4.21.83)" (EC 3.4.21.83)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.83

Use Curated BLAST to search for 3.4.21.83

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (708 amino acids)

>GFF146 Protease II (EC 3.4.21.83) (Sphingobium sp. HT1-2)
MIRTSAWLMASLSIAALSTTVEAQTAPKPPVAAKKPHEVKAPFGATRQDPYYWLRDDSRK
NPEMLAYLAAENAYADTLLAPTKPLQDKLYGEITGRIKQDDSSVPYRKKGYWYYSRFETG
GDYPIVARRKSNTDAPEQILLDEPRMGAGKGYFAIGGKEVSPDDRLLAYAEDLVGRRQYV
IRVKGLADGKLLSDEIPNAEPDFAWANDSKTIFYVEKDPTTLLGKRVKAHILGTPVSADR
LVYEEKDDSFYMGLKKTTSDKFICIVLQSTVSNEERCAPSANPASFTPLAPRERDFLYSS
DNVGGKWTIRTNWQAPNYRLMTISDADAAKGRAAWTDIVPTSASVFIENFQPFDSFLAIE
ERSEGNKRLRLLTKAGKSSFVNADEPAYAMALDVNAESGTPWVRYTYDSLRTPETTYEVN
VDTGERKILKVQEVKGYDPTKYVTERVWATARDGTKVPVSLIYAKGFQKNGSAPLYQYAY
GSYGYSTDPAFSPLLPSLLDRGVVYAIAHIRGGQEMGRQWYDDGHLKNKMNSFSDFIDVT
RALVDQGYAKKGRVAAMGGSAGGLLMGAVANMAPQDYKVIIAQVPFVDVVTTMLDPTIPL
TTGEYDEWGNPEQQSWYDVMLAYSPYDHVEAKAYPSLFVGTGLWDSQVQYYEPTKWVAKL
RATKTDSNPLVFRINMEAGHGGKSGRFRRYRDQAEYSAFALEQLGVAQ