Protein Info for GFF1459 in Variovorax sp. SCN45

Annotation: Glutathione S-transferase (EC 2.5.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13417: GST_N_3
  • amino acids 1 to 67 (67 residues), 34.6 bits, E=6.1e-12
• PF02798: GST_N
  • amino acids 1 to 65 (65 residues), 36.2 bits, E=1.8e-12
• PF13409: GST_N_2
  • amino acids 1 to 66 (66 residues), 45.8 bits, E=2.2e-15
• PF13410: GST_C_2
  • amino acids 113 to 176 (64 residues), 39.9 bits, E=1.1e-13
• PF00043: GST_C
  • amino acids 119 to 182 (64 residues), 45.3 bits, E=2.6e-15
• PF14497: GST_C_3
  • amino acids 120 to 186 (67 residues), 27.3 bits, E=1e-09

Best Hits

Swiss-Prot: 56% identical to YFCG_ECOLI: Disulfide-bond oxidoreductase YfcG (yfcG) from Escherichia coli (strain K12)

KEGG orthology group: K00799, glutathione S-transferase [EC: 2.5.1.18] (inferred from 76% identity to vpe:Varpa_2482)

MetaCyc: 56% identical to disulfide bond oxidoreductase YfcG (Escherichia coli K-12 substr. MG1655)
RXN0-6256

Predicted SEED Role

"Glutathione S-transferase (EC 2.5.1.18)" in subsystem Glutathione: Non-redox reactions (EC 2.5.1.18)

MetaCyc Pathways

• 4-hydroxy-2-nonenal detoxification (1/4 steps found)
• pentachlorophenol degradation (5/10 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• gliotoxin biosynthesis (1/9 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• indole glucosinolate activation (intact plant cell) (3/12 steps found)
• camalexin biosynthesis (2/12 steps found)

KEGG Metabolic Maps

• Drug metabolism - cytochrome P450
• Glutathione metabolism
• Metabolism of xenobiotics by cytochrome P450

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.18

Use Curated BLAST to search for 2.5.1.18

Protein Sequence (216 amino acids)

>GFF1459 Glutathione S-transferase (EC 2.5.1.18) (Variovorax sp. SCN45)
VHIMLEECGLPYAVHPVNIGAGDQFKPAFLAISPNNKIPAITDPLGPDGKPISLFESGAI
LLYLASKTGRFLPVSDRERFEVLQWLMFQVGGVGPMLGQTHHFRLYAAEKLPYAIERYSN
EALRLYGVIERRLASSRFIAGDDYSIADIALFPWLRSWESQGIVLGEFPHLKAWFEGIAV
RPAVQRALQVMADLRKPQRDPKEREVLFGKTQYQKR