Protein Info for GFF1458 in Sphingobium sp. HT1-2

Annotation: Replicative DNA helicase (DnaB) (EC 3.6.4.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 PF00772: DnaB" amino acids 18 to 118 (101 residues), 83.8 bits, see alignment E=1.2e-27 TIGR00665: replicative DNA helicase" amino acids 19 to 485 (467 residues), 489.1 bits, see alignment E=5.9e-151 PF03796: DnaB_C" amino acids 196 to 483 (288 residues), 337.6 bits, see alignment E=6.8e-105 PF13481: AAA_25" amino acids 197 to 387 (191 residues), 57.5 bits, see alignment E=2.1e-19

Best Hits

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 92% identity to sch:Sphch_0273)

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (495 amino acids)

>GFF1458 Replicative DNA helicase (DnaB) (EC 3.6.4.12) (Sphingobium sp. HT1-2)
MVELVKLNAAPEEGGLELPRNVEAEAALLGALMIDNRIAEDVQLKLKPEHFFEPLHGRIY
EAVMRLIERDMIANPVTLKPMLEQDPALKELGGAAYLAQLTGSGAALLGARDFATQIYDL
ALLRQLVNVGRELVDNALDTSEDVNPREQIELAEVALYKVSEGEGESGSVKSFLSASTMA
VQVAERALNSGGHVSGITTGINSLNEKIGGLHNSDLMILAGRPGMGKTSLATNIAYNAAS
RWLRDHSDGIPPEKNMGAKTAFFSLEMSADQLATRVLAEQSGISGEALRMGKISRADFQN
LSRAARDLQELPLFIDDTPGLTIAALRTRARRLKRRHDVGFIVVDYLQLLQGSGKAGDSR
VNEISEISRGLKTLAKELHVPVLALSQLSRAVEQREDKRPQLSDLRESGSIEQDADMVWF
VFREDYYVAAKEPGNKEGPEHQEWVQEMERIYGLAEVIVAKQRHGSTGRIRMKFDAKITR
FSDLAEGSSYLEDLE