Protein Info for GFF1457 in Xanthobacter sp. DMC5

Annotation: Malto-oligosyltrehalose trehalohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 583 TIGR02402: malto-oligosyltrehalose trehalohydrolase" amino acids 23 to 547 (525 residues), 634.9 bits, see alignment E=4.8e-195 PF00128: Alpha-amylase" amino acids 123 to 204 (82 residues), 39 bits, see alignment E=9.6e-14 PF11941: DUF3459" amino acids 493 to 572 (80 residues), 48.4 bits, see alignment E=1.5e-16

Best Hits

KEGG orthology group: None (inferred from 60% identity to bam:Bamb_5594)

Predicted SEED Role

"Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141)" in subsystem Trehalose Biosynthesis (EC 3.2.1.141)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.141

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (583 amino acids)

>GFF1457 Malto-oligosyltrehalose trehalohydrolase (Xanthobacter sp. DMC5)
MSGRGFDTTTAFGPILTANGTLFRLWAPGAEAIELELSSGTRLPMAPRAGGWFEAEAAVG
PGALYRFRLKDGTAIPDPASRAQAQDVHGFSIVTDPDAYRWRTDWAGRPWHEAVILEVHA
GLCGGFKGVAEKIPAWAALGITAVELMPVAAFPGTRNWGYDGVLPFAPAAIYGTPDELMA
LIDTAHAHGVMMLLDVVYNHFGPDGNYLGLYAPDFFHPEQATPWGPAVAFDRPEVSRFFL
ENALMWIEDFRFDGLRLDAVHTILPPQALAALGRDIRARLPADRPVHLVLENEHNDAALL
PRPFDAQWNDDIHHCLHVLLTGEREAYYGDFADAPAHRLARCLSEGFAYQGEAAPNLGRP
RGTPSAHLPPTAFVAFAQNHDQIGNRAFGERLLALADADGVRAALALVLLSPQVPMLFMG
EEMGARTPFLFFTDHAEGLADLVREGRRAEFARFSAFADPARRATIPDPNAQATFAASIP
VPPPDADEWQDFVEDMIAARQAHVVPRLAGAASLGAQAVSDHAVVARWRMGDGAVLTLAA
NFGGETCPADLPAALHLIVSSGAVEDFAIPPRATLAFLEGAPA