Protein Info for GFF1453 in Xanthobacter sp. DMC5

Annotation: Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1067 transmembrane" amino acids 895 to 912 (18 residues), see Phobius details PF11896: GlgE_dom_N_S" amino acids 422 to 604 (183 residues), 195.9 bits, see alignment E=1.1e-61 PF00128: Alpha-amylase" amino acids 615 to 753 (139 residues), 28.5 bits, see alignment E=1.4e-10 PF21702: GLGE_C" amino acids 974 to 1058 (85 residues), 61.5 bits, see alignment E=1.1e-20

Best Hits

Predicted SEED Role

"Alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1067 amino acids)

>GFF1453 Alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase (Xanthobacter sp. DMC5)
VHHQTMEGEYGRIPPVSDLTRPRLYHVPPRALPTGDARDAFFARIAALGFDTLLLGWPFP
VEPHSWFATRSLDTLAAPLDGERPEDAFRSLADVAAAHGLALFMDFACTHAGNSDLLAAL
PGCWSGVATAGDPRVSPATAGHSVTLADEAATARFTDFWAGHLDRWRRAGISGVRCHAAG
ALPAEVWQRLLGRARDAGLLALGWTPGLPRERVRELAPAFDLVTASSAWWDGRAPWLVEE
FNALRPHARLIGFCDDPFGPRLAARGPDATAEARHRLALAAAAQLADGWLMPMGFEHGAR
TPFMEADAGLDDAAGFDLSAQIAALNAAPVAVRLLRQLTSPGAAATLVKADGADGARLLA
FNPDLDHPAPLSVREAAARLGLALAGTAPDQLPPGGTAAIDLAATPPILAPRPAVAPFTS
APRIAIERVTPSVDGGRFAVKRCVGDMVTVEADIFADGHDMLSAELRWRAADEKTWQRAP
LVPTVNDRWQGRFPLLRVGHHDFLIEAWWDHFGTFRRDLGKKRAAGQKLAVEVSEGRALL
EEALERAPKGPRGVIRDHIRRLKDGEAAAVEALLSPALAAAMAEADPRAFATGATQLYPI
ECDREAAQFASWYELFPRSVTDDATRHGTLRDVMGRLPAIRDMGFDVLYFPPVHPIGRSH
RKGRNNALEAGPDDPGSPYAIGAEAGGHEAIHPELGTFDDFRALVAAARAEGLEIALDFA
VQCSPDHPWLKAHPDWFQWRPDGTIRYAENPPKKYEDIVNVDFYAKGAVPDLWLALRDVV
EGWVRLGVRIFRVDNPHTKPFPFWEWLIADIRSRDPGVIFLAEAFTRPKLMYRLGKIGFS
QSYTYFTWRNTKAELRDYLEELNRAPARDVYRPHFFVNTPDINPYFLQRSGRPGFLIRAA
LASMLSGLWGLYSGFELCEAAPVPGKEEYLDSEKYEIRPRDYSGPGNIVAEITRLNAIRR
AYPALQSHLNVTFYTAVDEQVLYFGKRLPDEDEMVLVAVSLDPHAPRSAAIEVPLWEFGL
PDHAAVEVEDLLRDTRFTWTGKMQTIHLDPADLPYAIWRIRPVGGVA