Protein Info for GFF1436 in Sphingobium sp. HT1-2

Annotation: S-formylglutathione hydrolase (EC 3.1.2.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 2 to 277 (276 residues), 414.7 bits, E=1e-128
• PF00756: Esterase
  • amino acids 21 to 271 (251 residues), 188.1 bits, E=1.3e-59

Best Hits

Swiss-Prot: 50% identical to ESTD_MOUSE: S-formylglutathione hydrolase (Esd) from Mus musculus

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 82% identity to sjp:SJA_C2-02870)

MetaCyc: 51% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of methylglyoxal degradation (4/8 steps found)
• superpathway of C1 compounds oxidation to CO₂ (5/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Protein Sequence (278 amino acids)

>GFF1436 S-formylglutathione hydrolase (EC 3.1.2.12) (Sphingobium sp. HT1-2)
VETVSTNTAFGGVQGVYRHASRETGTEMTFSVFVPPHAPGAKLPLVWYLSGLTCTHANVT
EKGEFRRACAELGLIFVAPDTSPRGEGVADDPDGAWDFGLGAGFYVDATQAPYAAHYRMW
SYVTQELPALIADQFPADMDRQSIMGHSMGGHGALTVGLTYPDRYRAVSAFAPIVAPGQV
PWGEKALGGYLGEDRAAWRKHDAVALIEDGARIDALLVDQGAGDSFLEGQLRPELLRTAC
DAAGIDLTLHLRDGYDHSYYFISSFMDDHLRWHAARLG