Protein Info for GFF1435 in Xanthobacter sp. DMC5

Annotation: Beta-ketoadipyl-CoA thiolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 71% identical to PCAF_PSEPU: Beta-ketoadipyl-CoA thiolase (pcaF) from Pseudomonas putida

KEGG orthology group: K00632, acetyl-CoA acyltransferase [EC: 2.3.1.16] (inferred from 85% identity to rhi:NGR_b21430)

MetaCyc: 73% identical to 3-oxoadipyl-CoA thiolase (Cupriavidus necator H16)
3-oxoadipyl-CoA thiolase. [EC: 2.3.1.174]

Predicted SEED Role

"Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ Beta-ketoadipyl CoA thiolase (EC 2.3.1.-)" (EC 2.3.1.-, EC 2.3.1.9)

MetaCyc Pathways

oleate β-oxidation (28/35 steps found)
phenylacetate degradation I (aerobic) (9/9 steps found)
superpathway of phenylethylamine degradation (10/11 steps found)
superpathway of salicylate degradation (7/7 steps found)
catechol degradation III (ortho-cleavage pathway) (6/6 steps found)
1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
adipate degradation (5/5 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
(2S)-ethylmalonyl-CoA biosynthesis (4/4 steps found)
acetyl-CoA fermentation to butanoate (6/7 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
ketolysis (3/3 steps found)
fatty acid salvage (5/6 steps found)
3-oxoadipate degradation (2/2 steps found)
acetoacetate degradation (to acetyl CoA) (2/2 steps found)
aromatic compounds degradation via β-ketoadipate (7/9 steps found)
adipate biosynthesis (4/5 steps found)
ketogenesis (4/5 steps found)
pyruvate fermentation to acetone (4/5 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
polyhydroxybutanoate biosynthesis (2/3 steps found)
L-isoleucine degradation I (4/6 steps found)
propanoate fermentation to 2-methylbutanoate (4/6 steps found)
pyruvate fermentation to butanol II (engineered) (4/6 steps found)
superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
glycerol degradation to butanol (11/16 steps found)
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
glutaryl-CoA degradation (3/5 steps found)
isopropanol biosynthesis (engineered) (3/5 steps found)
ethylmalonyl-CoA pathway (7/11 steps found)
pyruvate fermentation to hexanol (engineered) (7/11 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (12/18 steps found)
4-methylcatechol degradation (ortho cleavage) (4/7 steps found)
pyruvate fermentation to butanoate (4/7 steps found)
2-methyl-branched fatty acid β-oxidation (9/14 steps found)
5,6-dehydrokavain biosynthesis (engineered) (6/10 steps found)
L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (6/10 steps found)
valproate β-oxidation (5/9 steps found)
4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
2-deoxy-D-ribose degradation II (4/8 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
benzoyl-CoA degradation I (aerobic) (3/7 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
mevalonate pathway I (eukaryotes and bacteria) (3/7 steps found)
mevalonate pathway II (haloarchaea) (3/7 steps found)
3-phenylpropanoate degradation (5/10 steps found)
L-lysine fermentation to acetate and butanoate (5/10 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
4-ethylphenol degradation (anaerobic) (2/6 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
ethylbenzene degradation (anaerobic) (1/5 steps found)
fatty acid β-oxidation VII (yeast peroxisome) (1/5 steps found)
isoprene biosynthesis II (engineered) (3/8 steps found)
mevalonate pathway III (Thermoplasma) (3/8 steps found)
mevalonate pathway IV (archaea) (3/8 steps found)
pyruvate fermentation to butanol I (3/8 steps found)
toluene degradation III (aerobic) (via p-cresol) (5/11 steps found)
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
4-oxopentanoate degradation (3/9 steps found)
L-glutamate degradation VII (to butanoate) (5/12 steps found)
2-methylpropene degradation (2/8 steps found)
mandelate degradation to acetyl-CoA (9/18 steps found)
methyl tert-butyl ether degradation (2/10 steps found)
10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
L-tryptophan degradation III (eukaryotic) (4/15 steps found)
benzoate fermentation (to acetate and cyclohexane carboxylate) (5/17 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
jasmonic acid biosynthesis (4/19 steps found)
toluene degradation VI (anaerobic) (3/18 steps found)
cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
sitosterol degradation to androstenedione (1/18 steps found)
androstenedione degradation I (aerobic) (6/25 steps found)
superpathway of aerobic toluene degradation (9/30 steps found)
platensimycin biosynthesis (6/26 steps found)
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
superpathway of ergosterol biosynthesis I (5/26 steps found)
superpathway of testosterone and androsterone degradation (6/28 steps found)
superpathway of aromatic compound degradation via 3-oxoadipate (11/35 steps found)
Methanobacterium thermoautotrophicum biosynthetic metabolism (25/56 steps found)
androstenedione degradation II (anaerobic) (4/27 steps found)
superpathway of L-lysine degradation (13/43 steps found)
superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
superpathway of cholesterol biosynthesis (5/38 steps found)
superpathway of cholesterol degradation II (cholesterol dehydrogenase) (9/47 steps found)
superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-, 2.3.1.16, 2.3.1.174, 2.3.1.9

Use Curated BLAST to search for 2.3.1.- or 2.3.1.16 or 2.3.1.174 or 2.3.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (400 amino acids)

>GFF1435 Beta-ketoadipyl-CoA thiolase (Xanthobacter sp. DMC5)
MKDVFICDYVRTPIGRFGGALASVRADDLGAVPLKALMARNPGVDFGAVDDVIFGCANQA
GEDNRNVARMSLLLAGLPVGVPGTTINRLCGSGMDAIIAAARAIKAGEASLMIAGGVESM
SRAPFVMPKADTAFSRHAEIHDTTIGWRFVNPLMKAQYGVDSMPETGENVAEDFAINRAD
QDAFALRSQAKAAAAQANGRLAREIVPVAIPQRKGDPILVERDEHPRATTIEALAKLPTP
FRKGGSVTAGNASGVNDGAAALILADAETAAKYGLTPIARVLGGAAAGVPPRIMGIGPAP
ASKKLMARLGLTPADFDVIELNEAFASQGLATLRDLGIADDDARVNPNGGAIALGHPLGM
SGARITGTAAIELSLTGGRRSLSTMCIGVGQGIAVALERV