Protein Info for PS417_00720 in Pseudomonas simiae WCS417

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details PF16576: HlyD_D23" amino acids 46 to 237 (192 residues), 42.2 bits, see alignment E=9e-15 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 46 to 215 (170 residues), 117.1 bits, see alignment E=4e-38 PF13533: Biotin_lipoyl_2" amino acids 48 to 94 (47 residues), 52.8 bits, see alignment 4.1e-18 PF13437: HlyD_3" amino acids 155 to 237 (83 residues), 47.3 bits, see alignment E=4.8e-16

Best Hits

Swiss-Prot: 42% identical to YDHJ_ECOLI: Uncharacterized protein YdhJ (ydhJ) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 98% identity to pfs:PFLU0139)

MetaCyc: 40% identical to aromatic carboxylic acid efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-233

Predicted SEED Role

"HlyD family secretion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UE50 at UniProt or InterPro

Protein Sequence (296 amino acids)

>PS417_00720 membrane protein (Pseudomonas simiae WCS417)
MKKPFLTIGRVVLTLLIVTFAVVVVWRMVMYYMFAPWTRDGHIRADIVQIAPDVSGLIQR
VDVRDNQLVTKGQVLFAVDQDRFKLALRQAQAAVADRQETLAQAQREYKRNRGLGNLVPS
EQLEESQSRVARAQSALAEAQVTVDSAQLNLDRSVIRSPVDGYVNDRAPRTQEFVTAGRP
VLSVVDSNSFHIDGYFEETKLDGIHVGMGVDIRVIGDNARLHGHVQSIVAGIEDRDRSSG
SNLLPNVNPAFSWVRLAQRIPVRIAFDDVPADFRMIAGRTATVSIIGDKVKDGDKP