Protein Info for GFF143 in Variovorax sp. SCN45

Annotation: Dethiobiotin synthase BioD (EC 6.3.3.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 248 signal peptide" amino acids 18 to 33 (16 residues), see Phobius details transmembrane" amino acids 135 to 157 (23 residues), see Phobius details amino acids 166 to 185 (20 residues), see Phobius details PF13500: AAA_26" amino acids 4 to 213 (210 residues), 135 bits, see alignment E=3.2e-43 TIGR00347: dethiobiotin synthase" amino acids 7 to 176 (170 residues), 158.6 bits, see alignment E=7.4e-51 PF01656: CbiA" amino acids 7 to 215 (209 residues), 29.4 bits, see alignment E=6.9e-11

Best Hits

Swiss-Prot: 58% identical to BIOD_AROAE: ATP-dependent dethiobiotin synthetase BioD (bioD) from Aromatoleum aromaticum (strain EbN1)

KEGG orthology group: K01935, dethiobiotin synthetase [EC: 6.3.3.3] (inferred from 82% identity to vap:Vapar_2812)

Predicted SEED Role

"Dethiobiotin synthetase (EC 6.3.3.3)" in subsystem Biotin biosynthesis (EC 6.3.3.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.3.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (248 amino acids)

>GFF143 Dethiobiotin synthase BioD (EC 6.3.3.3) (Variovorax sp. SCN45)
MTPRRWFVTGTDTGIGKTLVSSAMLSVLAASGLRAVGMKPVAAGLEQIDGEWCNEDVTRL
HEAGNVDAPLALRCPYLLRAPMSPHLAAREEGMRIALQPLLSAFEQLSARADAVVVEGVG
GFCVPFGDDLDSADLAVALGLPVILVVGVRLGCLNHALLTAEAIRARGLVLAGWVASMID
PLMLAPQANLQTLRARLGAPLLGIVPHLEHANAAQAARHIDLRALRATAAHAARLAALQA
PEVSAALA