Protein Info for GFF1429 in Xanthobacter sp. DMC5

Annotation: Butyrate--acetoacetate CoA-transferase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02428: 3-oxoacid CoA-transferase, B subunit
  • amino acids 15 to 218 (204 residues), 287.4 bits, E=2.5e-90
• PF01144: CoA_trans
  • amino acids 17 to 211 (195 residues), 133.1 bits, E=5e-43

Best Hits

Swiss-Prot: 55% identical to CTFB_CLOAB: Butyrate--acetoacetate CoA-transferase subunit B (ctfB) from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)

KEGG orthology group: K01029, 3-oxoacid CoA-transferase subunit B [EC: 2.8.3.5] (inferred from 69% identity to msl:Msil_1856)

MetaCyc: 55% identical to butyrate-acetoacetate CoA-transferase, subunit B (Clostridium acetobutylicum ATCC 824)
Acetoacetyl-CoA hydrolase. [EC: 3.1.2.11]; Butyrate--acetoacetate CoA-transferase. [EC: 3.1.2.11, 2.8.3.9]; 2.8.3.9 [EC: 3.1.2.11, 2.8.3.9]

Predicted SEED Role

"Acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (EC 2.8.3.8)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 2.8.3.8)

MetaCyc Pathways

• acetyl-CoA fermentation to butanoate (6/7 steps found)
• ketolysis (3/3 steps found)
• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• pyruvate fermentation to acetone (4/5 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• isopropanol biosynthesis (engineered) (3/5 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (3/7 steps found)
• L-lysine fermentation to acetate and butanoate (5/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)
• succinate fermentation to butanoate (2/7 steps found)
• superpathway of L-lysine degradation (13/43 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Propanoate metabolism
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 2.8.3.5, 2.8.3.8

Use Curated BLAST to search for 2.8.3.5 or 2.8.3.8 or 2.8.3.9 or 3.1.2.11

Protein Sequence (229 amino acids)

>GFF1429 Butyrate--acetoacetate CoA-transferase subunit B (Xanthobacter sp. DMC5)
MDAKVMDRPRAGMSAKEIIARRVAQEIKPGMLVNLGIGLPSMVANYIPHDYGVYFQAENG
VIGLGARPPEGMEDPHLTDAGGGFVTAVPGAASIDSAMSFGIIRGGHLDMTVLGGLQVDE
RGYLANWMVPGKMVPGMGGAMDLVAGAKRVVVAMVHSAKGEAKIVPECTLPLTAMRRVSL
IVTELAVIEPTREGLLLRERGPDVRVEDILAQTSARLIIEDEVPEMRIG