Protein Info for GFF1427 in Variovorax sp. SCN45

Annotation: Mandelate racemase (EC 5.1.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02746: MR_MLE_N
  • amino acids 9 to 128 (120 residues), 72.7 bits, E=3e-24
• PF13378: MR_MLE_C
  • amino acids 151 to 351 (201 residues), 187.5 bits, E=2.8e-59

Best Hits

Swiss-Prot: 57% identical to MANR_PSEPU: Mandelate racemase (mdlA) from Pseudomonas putida

KEGG orthology group: K01781, mandelate racemase [EC: 5.1.2.2] (inferred from 60% identity to bxe:Bxe_B0135)

MetaCyc: 57% identical to mandelate racemase (Pseudomonas putida)
Mandelate racemase. [EC: 5.1.2.2]; 5.1.2.2 [EC: 5.1.2.2]

Predicted SEED Role

No annotation

MetaCyc Pathways

• 4-hydroxymandelate degradation (3/6 steps found)
• mandelate degradation I (2/5 steps found)
• mandelate degradation to acetyl-CoA (9/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (21/35 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (17/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation
• Toluene and xylene degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.2.2

Protein Sequence (358 amino acids)

>GFF1427 Mandelate racemase (EC 5.1.2.2) (Variovorax sp. SCN45)
MSELKIKGVRARAVNVPLEYPVRTSVGVVATSPLVLIDLETNVGVTGRAYVFTYTPLALA
ATRCLVETLGSAIVGRPVAPLDIDAYFAGRLRLLGKTGMTAMACAGLDMALWDALAVHQG
VPLVTLLGGTPKQIPAYDSHSMDGQEVGVLRARKSVSQGFRAIKTKIGYPTLERDLEVVR
ALRQAIGQGVELMADYNQGLTAPEAARRIRALADEGISWIEEPIIQEDLAGHARLRSAGL
PIQMGENWCGPEQMQLALAAGACDLAMPDAMKIGGVTGWMRAAALGQAAGIPMSSHIFQE
VSAHLLSVTPTAHWLERMDLAGPVLDGGLRFEEGFAKCPELPGTGIAWNERAVAQYAL