Protein Info for GFF1421 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: Replicative DNA helicase (EC 3.6.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 TIGR00665: replicative DNA helicase" amino acids 27 to 464 (438 residues), 579.4 bits, see alignment E=2.3e-178 PF00772: DnaB" amino acids 27 to 128 (102 residues), 128.4 bits, see alignment E=1.6e-41 PF03796: DnaB_C" amino acids 206 to 462 (257 residues), 359.4 bits, see alignment E=1.5e-111 PF13481: AAA_25" amino acids 222 to 385 (164 residues), 36.9 bits, see alignment E=4.7e-13

Best Hits

Swiss-Prot: 100% identical to DNAB_SALTY: Replicative DNA helicase (dnaB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 100% identity to ses:SARI_03432)

MetaCyc: 93% identical to replicative DNA helicase (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (471 amino acids)

>GFF1421 Replicative DNA helicase (EC 3.6.1.-) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MAGNKPFNKPQTDARDRDPQVAGIKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVAEDF
YTRPHRHIFTEMGRLQESGSPIDLITLAESLERQGQLDSVGGFAYLAELSKNTPSAANIS
AYADIVRERAVVRDMIAVAHEIADAGYDPQGRNSDELLDLAESRVFQIAENRANKDEGPK
SIDQILDATVARIEQLFQQPHDGVTGVDTGYQDLNKKTAGLQRSDLIIVAARPSMGKTTF
AMNLCENAAMLQDKPVLIFSLEMPGEQIMMRMLASLSRVDQTRIRTGQLDDEDWARISGT
MGILLEKRNMYIDDSSGLTPTEVRSRARRIFREHGGLSLIMIDYLQLMRVPSLSDNRTLE
IAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYR
DEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPQYDDE