Protein Info for GFF142 in Xanthobacter sp. DMC5

Annotation: Cytosol aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 498 transmembrane" amino acids 370 to 388 (19 residues), see Phobius details PF02789: Peptidase_M17_N" amino acids 21 to 146 (126 residues), 75.3 bits, see alignment E=4.2e-25 PF00883: Peptidase_M17" amino acids 183 to 488 (306 residues), 423.3 bits, see alignment E=6.2e-131

Best Hits

Swiss-Prot: 87% identical to AMPA_XANP2: Probable cytosol aminopeptidase (pepA) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 87% identity to xau:Xaut_1462)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.1

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (498 amino acids)

>GFF142 Cytosol aminopeptidase (Xanthobacter sp. DMC5)
MSDPVTVSFARLTAAAKGVLVVLTDESLAFGTQAQKLVKGADKALTRAFDAEKFKGKAWS
ALDLLAPAGIEAPRLIVISVGKAADLKPKDFLKLGGLIAGKLPASAREASVVLDLPGTKI
SADAAAEVALGISLRSYSFDRYKTKKKDDAEPTARAVTLLVSDEAGTKRSYAARQAVGDG
VLFARDLVNEPANVLDPPEFARRAEEHLSGVGVEIEVLDDAALAEAGLKALLGVGQGSIK
ESRVVVMRWNGGPKDEAPVAFIGKGVTFDTGGISIKPAAGMEDMKGDMGGAACVTGLMYA
LAARKAKVNAVGLIGLVENMPDGAAQRPGDIVTSLSGQTIEIINTDAEGRLVLCDVLWYA
KERFKPKFMIDLATLTGAILVALGSEYAGLFSNDDELSARLTQAGQETGERVWRMPLGPE
YDKLIDSKFADMKNTGGRNAGSITAAQFLHRFVDKTPWAHLDVAGTAMSSPASDINKSWG
SGWGVRLLDQLVKDHYEG