Protein Info for GFF1409 in Sphingobium sp. HT1-2

Annotation: Nicotinate phosphoribosyltransferase (EC 6.3.4.21)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 TIGR01514: nicotinate phosphoribosyltransferase" amino acids 20 to 421 (402 residues), 293.1 bits, see alignment E=1.8e-91 PF17767: NAPRTase_N" amino acids 25 to 149 (125 residues), 91.8 bits, see alignment E=4.3e-30 PF04095: NAPRTase" amino acids 191 to 422 (232 residues), 145.4 bits, see alignment E=2.5e-46

Best Hits

Swiss-Prot: 78% identical to PNCB_NITHX: Nicotinate phosphoribosyltransferase (pncB) from Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)

KEGG orthology group: K00763, nicotinate phosphoribosyltransferase [EC: 2.4.2.11] (inferred from 78% identity to nha:Nham_3403)

Predicted SEED Role

"Nicotinate phosphoribosyltransferase (EC 2.4.2.11)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation or Redox-dependent regulation of nucleus processes (EC 2.4.2.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>GFF1409 Nicotinate phosphoribosyltransferase (EC 6.3.4.21) (Sphingobium sp. HT1-2)
MVFTDIATRTYNHNWRLDPIVRSLLDTDFYKLLMLQMIRQQHPDVQTTFKLINRTHHVRL
ADIIDEGELRAQLDHARSLRFGKKELIWLAGNSFYGRTQMFSPDFIAWLADFRLPEYELR
QVDGQYELRFHGLWIETMMWEIPALAIINELRSRAAMKGRGRFALDVLYARAKAKLWEKV
ERLSALPDLVISDFGTRRRHGFLWQRWCVEALKEGLGDRFIGSSNVLLAMDADLEAIGTN
AHELPMVLAALALDDAALARTPYTVLEQWQAHYGGNLRIALPDAFGTAAFLKDAPDWVAD
WTGFRPDSAPPIEAGEQIIRWWQEKGRDPRSKLLIFSDGMDVDSIEATYRHFHGRVRMSF
GWGTNLTNDFRGCAPFETDGLDPISLVCKVVDANGRPAVKLSDNPAKATGDPAEIDRYLR
VFGGDGRKAVAVQV