Protein Info for PS417_07090 in Pseudomonas simiae WCS417

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 172 to 194 (23 residues), see Phobius details PF08521: 2CSK_N" amino acids 19 to 167 (149 residues), 139.7 bits, see alignment E=1.4e-44 PF00672: HAMP" amino acids 192 to 239 (48 residues), 29.1 bits, see alignment 2e-10 PF00512: HisKA" amino acids 245 to 309 (65 residues), 57.7 bits, see alignment E=2e-19 PF02518: HATPase_c" amino acids 358 to 457 (100 residues), 76.1 bits, see alignment E=5.7e-25

Best Hits

KEGG orthology group: K07649, two-component system, OmpR family, sensor histidine kinase TctE [EC: 2.7.13.3] (inferred from 98% identity to pfs:PFLU1452)

Predicted SEED Role

"Tricarboxylate transport sensor protein TctE"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U6B0 at UniProt or InterPro

Protein Sequence (461 amino acids)

>PS417_07090 histidine kinase (Pseudomonas simiae WCS417)
MHKPSSLRWRLLWNLALLLVLLMLASGMSAYWNGREAADTAYDRTLLASARTIAAGLTQV
DGTLSANVPYVALDTFAYDSAGRIYYQVNDIHQKLISGYENLPGPPPGTPRTDDYPALAR
FYDAMYQGQQVRVVSLLKAVSEPNMNGMAEIRVAETDEARVSMARSLMADTLLRLGMLAV
GALLLVWFAVSAALRPLERLRTAVEERQPDDLRPLPLVEVQHEFGPLVRSLNHFTERLRG
QFERQAQFIADAAHELRTPLAALKARLELGLRAEDPATWRSTLETAAQGTDRLTHLANQL
LSLARIENGARAIAEGGAQLLDLSQLARELGMAMAPLAHARGVALALEADEPVWLRGEPT
LLNELLSNLVDNALAHTSPGGNVILRVTAPAVLEVEDDGPGIPLDERDRVFERFYRRSQQ
GMGSGLGLAIVGEICRAHLAQISLHDGEQAGLKVRVSFIAG