Protein Info for GFF1394 in Sphingobium sp. HT1-2

Annotation: Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 691 PF00378: ECH_1" amino acids 12 to 198 (187 residues), 134.3 bits, see alignment E=1e-42 PF16113: ECH_2" amino acids 16 to 183 (168 residues), 64.5 bits, see alignment E=2.6e-21 PF02737: 3HCDH_N" amino acids 301 to 478 (178 residues), 213.3 bits, see alignment E=5.3e-67 PF00725: 3HCDH" amino acids 482 to 566 (85 residues), 55.9 bits, see alignment E=1.1e-18 amino acids 602 to 686 (85 residues), 33.3 bits, see alignment E=1.1e-11

Best Hits

KEGG orthology group: K07516, 3-hydroxyacyl-CoA dehydrogenase [EC: 1.1.1.35] (inferred from 87% identity to sch:Sphch_0602)

MetaCyc: 53% identical to 6-carboxyhex-2-enoyl-CoA hydratase (Rhodopseudomonas palustris)
RXN-8031

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3, EC 5.3.3.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.1.2.3

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3 or 5.3.3.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (691 amino acids)

>GFF1394 Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) (Sphingobium sp. HT1-2)
MKVNDVVTYAVEGSIAVITVNSPPVNALSNAVRQGVASAVETAIGDPAVGAMVLICEGRT
FFAGADITEFGKPPVEPTLRNLQLVVENATKPVIDAIHGTALGGGLELALVAHYRIAVPS
AKCGLPEVNLGLLPGAGGTQRLPRIVGVEKALDLMTAGSPVSAKVAKEMGLIDELAAEGQ
LRTDAITFAQRVLDEGRPLLKVRDRTEKLDEARGKPEIFADFRKANARKFRGFNAPEAII
RCVEASLELPFDEGMTFERQLFNELQADSQSAAQRHVFFAERQAAKVDSLPADTAILPIA
KVGVIGAGTMGGGIAMNFANAGIPVTLVETNEDALFRGLRIIRGNYETTAKKGKMKPEDV
DTRMGLLTGSIAMEDLADCDLIIEAVFEQIDVKREVFGKLDRIAKPGAILATNTSYLDID
DIAAATSRPDSVVGLHFFSPANVMKLLEVVRTKDTKPEIIATAMKLAKTIGKIGVLVGNG
YGFVGNRILAARNTQADQLILEGATPWAVDKVLYDFGFAMGHFQMRDLVGLDVGWNPKTT
SSANVREILNEMGRHGQKTKGGFYDYDENRKQTPSPVAMQVIEDFAAKQGIVRRDVSDAE
IQDRILFAMVNEGARILDEGIASRASDVDIVWITGYGWPKYRGGPMFWADLQGLPTVLDR
LKALEAAHGAAFTPSPLIERLVAQGKGFKDA