Protein Info for GFF1393 in Sphingobium sp. HT1-2

Annotation: Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.2.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 695 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 25 to 539 (515 residues), 491.4 bits, see alignment E=1.6e-151 PF01011: PQQ" amino acids 30 to 349 (320 residues), 193.9 bits, see alignment E=1.1e-60 amino acids 382 to 534 (153 residues), 36 bits, see alignment E=7.1e-13 PF13360: PQQ_2" amino acids 58 to 205 (148 residues), 62.2 bits, see alignment E=1.2e-20 amino acids 189 to 352 (164 residues), 23.1 bits, see alignment E=1.1e-08 amino acids 460 to 526 (67 residues), 25.7 bits, see alignment E=1.7e-09 PF13442: Cytochrome_CBB3" amino acids 594 to 666 (73 residues), 39.6 bits, see alignment E=1.1e-13

Best Hits

KEGG orthology group: None (inferred from 87% identity to sch:Sphch_0603)

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.8

Use Curated BLAST to search for 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (695 amino acids)

>GFF1393 Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.2.8) (Sphingobium sp. HT1-2)
MRLASLTLPFLALLAACAPSGQTRRDGTDWPGYGGIDENHYSPLKDINGHNVGRLGLAWY
QDIDGGGSSLTAPIAVDGILYYASGYSVVHAVDAATGHELWTYDPQSWKVADQKMRGAWG
SRGIAYDNGAVYVGTIDGRLIAINARTGHRLWSTQTIGKDDERYISGAPWVFNGKVLIGH
GGADFAPIRGYVTAYDQKTGKQLWRFYTVPGDPKLGFENKAMAMAAKTWTGEWWKYGGGG
TAWNAMAYDPKYNRIYIGVGNGSPWNQKIRSPGGGDNLFLCSIVALDADTGEYVWHYQTN
PGETWDFNSAMDMELARLKIDGRERDVLMHAPKNGFFYVIDRATGKLISARNIVPVNWAS
GIDVKSGRPIENPAARYPGGKAAIVYPSPFGAHNIEAMSFNPGSGLVYIPTMDQGRVYID
PAEPLESWKHLDGQRLSVGTGAPPPGVTPDRPATSFLLAWNPVTQSEAWRIPMPGLRGGG
GTATTAGNLLFQGNAGGKFVAYAATSGKPLWSFDAQTAVMAQPISYRARGRQYVTVIAGS
RFPSAIGLPREWNYRTQQWRVLTFALDGKAALPKVDPVDMPVIDDPAFAVDPAKAAIGAT
VFGQRCSICHGANAVSGGAAPDLLQSGVPLDTASMKDVLHNGILRERGMPRFQELTDDEI
AGLQHYFRQRARQVLAAQSAGQPGAQTHRGLNEGQ