Protein Info for GFF1386 in Sphingobium sp. HT1-2

Annotation: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 1 / 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 TIGR03550: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofG subunit" amino acids 43 to 366 (324 residues), 465.3 bits, see alignment E=1.5e-143 PF04055: Radical_SAM" amino acids 50 to 229 (180 residues), 51.1 bits, see alignment E=1.9e-17 amino acids 459 to 633 (175 residues), 77.5 bits, see alignment E=1.4e-25 TIGR03551: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunit" amino acids 417 to 763 (347 residues), 511.5 bits, see alignment E=1.4e-157 TIGR00423: radical SAM domain protein, CofH subfamily" amino acids 451 to 762 (312 residues), 372.9 bits, see alignment E=1.9e-115 PF19288: CofH_C" amino acids 646 to 762 (117 residues), 45.7 bits, see alignment E=5.9e-16

Best Hits

KEGG orthology group: K11779, FO synthase [EC: 2.5.1.-] (inferred from 88% identity to sch:Sphch_0610)

Predicted SEED Role

"7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 1 / 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2" in subsystem Coenzyme F420 synthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-

Use Curated BLAST to search for 2.5.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (805 amino acids)

>GFF1386 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 1 / 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 (Sphingobium sp. HT1-2)
MAVTERIGEAEHARTAELLAMPLAELMAQAAALRDEGHGRVQSWSRKIFIPLTQLCRNLC
HYCTFSQPPRPGENAYMTREDVLAVARAGKAAGCTEALFTLGDKPELRFAAAREDLARLG
HDSTLSYLAEMAALVRDETGLLPHINAGVMTREEMAGLRRVSVSQGLMLESVSDRLCRKG
GAHYRSPDKEPAARLETIRLAGELAIPFTTGILIGIGETREERIEALEAIRDLQDGYGHI
QEVIVQNFRAKERTVMANAPEPDMDDLLWTIAVARLILGPEMNIQAPPNLSAADFPRLMA
AGINDWGGVSPVTPDHVNPEAPWPAVERLRAATEGEGRMLVARLPVYPAYAQDNERWQDQ
AMVRHVLAHADAMGFAREDSWSPGIALEPRVTRPSALPVDPAIAAIVDRAMAGALLEEDE
VETLFSARETDLEHVCASADALRMQEMGDTISYVVNRNINYTNICAYKCSFCAFSKGDKA
EALRGKPYDLDLEEVVRRTREAWERGGTEVCLQGGIHPHYTGDTYLNLARAVKAAVPEMH
IHAFSPLEVWQGAATLGLPVEDFLRRLKAVGLDTLPGTAAEILDDEVRAIICPDKLDTAQ
WLEVIETAHRVGFTTTATIMFGHVDTPRHWARHLIRIRDLQRRTGGFTEFVPLPFVHMEA
PMGVRGQSRHGPTFREVRLMHAVARLALHPHIRSIQTSWVKLGPEGVKACLEGGANDLGG
TLMNESISRAAGTQHGQEMPPAAMEALIRSIDRTPRQRSTAYGGVSEDIHARGMTAAELT
PMVLTPPRKRRREADTNQERFEYAE