Protein Info for GFF1385 in Variovorax sp. SCN45

Annotation: Multidrug resistance transporter, Bcr/CflA family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 404 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 44 to 64 (21 residues), see Phobius details amino acids 76 to 94 (19 residues), see Phobius details amino acids 100 to 121 (22 residues), see Phobius details amino acids 133 to 158 (26 residues), see Phobius details amino acids 164 to 183 (20 residues), see Phobius details amino acids 213 to 232 (20 residues), see Phobius details amino acids 246 to 266 (21 residues), see Phobius details amino acids 278 to 299 (22 residues), see Phobius details amino acids 305 to 331 (27 residues), see Phobius details amino acids 342 to 363 (22 residues), see Phobius details amino acids 369 to 390 (22 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 7 to 385 (379 residues), 304.1 bits, see alignment E=1e-94 PF07690: MFS_1" amino acids 15 to 356 (342 residues), 159.3 bits, see alignment E=1.3e-50 amino acids 237 to 389 (153 residues), 34.1 bits, see alignment E=1.5e-12 PF00083: Sugar_tr" amino acids 45 to 182 (138 residues), 31 bits, see alignment E=1.3e-11

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 96% identity to vpe:Varpa_3138)

Predicted SEED Role

"MFS family multidrug efflux protein, similarity to bicyclomycin resistance protein Bcr"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (404 amino acids)

>GFF1385 Multidrug resistance transporter, Bcr/CflA family (Variovorax sp. SCN45)
MRSSPFFKMALLLGLLSAIGPFAIDMYLPALPAIGQSLHADIGAVQMSLTAFFLSLGAGQ
LLYGPISDMVGRKPPLYAGLVLFALASVGCAVATDIQTLIVLRFVQGLGAAAGMAIPRAV
VRDLHTGTDAARLMSLLMLVFSVSPILAPLAGSAVIAFAGWRGVFWAVTIAAVAGLAMMV
KLLDETRPASERVESSLGSALAAYWLLLRDRHYLGLVFIGSFAMAGFFTYLANSSFVMID
HYGLSPAMYSVAFGVNAAAFIAASQFTGSLGERYGLVNLVKFGVVCCGVAMVAMFIYFAT
GGDNIWVLIVLYFIASGFMGLVIPTTGVLALEMHGAIAGTASALLGTLQMLTGALAMAVV
GLFTDGRPLPMVAGMAAGALIALVLTWLTLGGVRSEPAARAQQA