Protein Info for GFF1384 in Sphingobium sp. HT1-2

Annotation: Coenzyme F420-0:L-glutamate ligase (EC 6.3.2.31) @ F420-1:L-glutamate ligase (EC 6.3.2.34)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 257 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR01916: coenzyme F420-0:L-glutamate ligase" amino acids 11 to 256 (246 residues), 260.4 bits, see alignment E=7.8e-82 PF01996: F420_ligase" amino acids 16 to 234 (219 residues), 247.8 bits, see alignment E=4.4e-78

Best Hits

Swiss-Prot: 47% identical to COFE_METTH: Coenzyme F420:L-glutamate ligase (cofE) from Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)

KEGG orthology group: K12234, coenzyme F420-0:L-glutamate ligase / coenzyme F420-1:gamma-L-glutamate ligase [EC: 6.3.2.31 6.3.2.34] (inferred from 69% identity to swi:Swit_0415)

Predicted SEED Role

"Coenzyme F420-0:L-glutamate ligase @ Coenzyme F420-1:L-glutamate ligase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.31 or 6.3.2.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (257 amino acids)

>GFF1384 Coenzyme F420-0:L-glutamate ligase (EC 6.3.2.31) @ F420-1:L-glutamate ligase (EC 6.3.2.34) (Sphingobium sp. HT1-2)
MIVAPAAVTLSALNGVPMVAPGDDLSAMLLSACHANGLSLQDGDIVVLAQKIISKAEGRA
VPLASVSPSAEAIDLAAQCHKDPRLVELILSESEKLMRVRPGVLIVRHRLGLVLANAGID
QSNIAQDGDGVALLLPLDPDGSAAALRRTVREALGVDIAIVIIDSLGRAWRKGTCGVAIG
VAGMSGLLDLRGEPDLHGRPLQTSELGLADEVAAAASLIMGQAGEGRPIVVARGIARGRN
EGSAAELIREPAMDLFP