Protein Info for GFF1383 in Xanthobacter sp. DMC5

Annotation: 1,2-epoxyphenylacetyl-CoA isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02280: phenylacetate degradation probable enoyl-CoA hydratase PaaB
  • amino acids 15 to 264 (250 residues), 403.4 bits, E=2e-125
• PF00378: ECH_1
  • amino acids 19 to 263 (245 residues), 213 bits, E=4.7e-67
• PF16113: ECH_2
  • amino acids 21 to 201 (181 residues), 99.4 bits, E=3.2e-32

Best Hits

Swiss-Prot: 61% identical to PAAG_ECOLI: 1,2-epoxyphenylacetyl-CoA isomerase (paaG) from Escherichia coli (strain K12)

KEGG orthology group: K01692, enoyl-CoA hydratase [EC: 4.2.1.17] (inferred from 83% identity to xau:Xaut_0903)

MetaCyc: 65% identical to 1,2-epoxyphenylacetyl-CoA isomerase (Pseudomonas sp. Y2)
RXN0-6510 [EC: 5.3.3.18]

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17)" in subsystem Acetyl-CoA fermentation to Butyrate or Butanol Biosynthesis or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation (EC 4.2.1.17)

MetaCyc Pathways

• oleate β-oxidation (28/35 steps found)
• phenylacetate degradation I (aerobic) (9/9 steps found)
• superpathway of phenylethylamine degradation (10/11 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• adipate degradation (5/5 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• adipate biosynthesis (4/5 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• L-isoleucine degradation I (4/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• glycerol degradation to butanol (11/16 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (12/18 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• valproate β-oxidation (5/9 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• benzoyl-CoA degradation I (aerobic) (3/7 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• 3-phenylpropanoate degradation (5/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• pyruvate fermentation to butanol I (3/8 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• L-glutamate degradation VII (to butanoate) (5/12 steps found)
• L-tryptophan degradation III (eukaryotic) (4/15 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (5/17 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (4/16 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.17

Use Curated BLAST to search for 4.2.1.17 or 5.3.3.18

Protein Sequence (266 amino acids)

>GFF1383 1,2-epoxyphenylacetyl-CoA isomerase (Xanthobacter sp. DMC5)
VEAHLNEAVLTVTDHGGWTELTLNRPDRLNSFNEEMHGALAAALDAAADESCRAVLLTGA
GRGFCAGQDLSARANAEGPLDLGATIEAFYNPLIRRIRGLHKPVICAVNGVAAGAGANIA
LACDIVLAARSAKFIQAFSKIGLVPDSGGTYFLPRLIGDARARALMLLAEPLAAETAAEW
GLIWKVIDDAALMDEARALTARLATQPTQGLALTKAALNTSGGNSLNDQLDLERDLQREA
GRTPDYREGVKAFMEKRAPNFTGRRS