Protein Info for GFF1345 in Variovorax sp. SCN45

Annotation: 3-methylmercaptopropionyl-CoA dehydrogenase (DmdC)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 617 PF12418: AcylCoA_DH_N" amino acids 7 to 30 (24 residues), 26.4 bits, see alignment (E = 1.7e-09) PF02771: Acyl-CoA_dh_N" amino acids 35 to 144 (110 residues), 22.9 bits, see alignment E=2.8e-08 PF02770: Acyl-CoA_dh_M" amino acids 157 to 243 (87 residues), 35.1 bits, see alignment E=3.6e-12 PF00441: Acyl-CoA_dh_1" amino acids 353 to 474 (122 residues), 60.8 bits, see alignment E=5.4e-20 PF08028: Acyl-CoA_dh_2" amino acids 386 to 467 (82 residues), 23.2 bits, see alignment E=2.1e-08 PF12806: Acyl-CoA_dh_C" amino acids 492 to 613 (122 residues), 96.1 bits, see alignment E=5.2e-31

Best Hits

KEGG orthology group: K00248, butyryl-CoA dehydrogenase [EC: 1.3.8.1] (inferred from 93% identity to vpe:Varpa_3182)

Predicted SEED Role

"3-methylmercaptopropionyl-CoA dehydrogenase (DmdC)"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.8.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (617 amino acids)

>GFF1345 3-methylmercaptopropionyl-CoA dehydrogenase (DmdC) (Variovorax sp. SCN45)
MSLRSTLDFLLYDWLDAESLNQRDRFADHSRETFDAVLDICERIAREKYAPFNRTVDTQE
PHFDGEKVILPQATHDAHKAFVESGMMSAAQDYDIGGMQLPYTMQAAANSFFAMASVSIG
SNMLTSGNANLLMVHGTDMQKNVFAKNEFSGRWAGTMCLSEPQAGSSLSDVATRAVPDGE
GFQDDPLGPRYRLTGNKMWISAGDHELTENIVHIVLAKIPDENGKLVPGTRGISLFIVPK
RMVDTKGKLTGQRNDVALAGLNHKLGWRGTTNTLLNFGEGKYPVDGKAGAVGYLVGEPGK
GLHCMFHMMNEARIGVGTAATMLGMAGYHASLEYAKTRPQGRLVKKPQAAGTAVAKDSAA
PQVRIIEHADVRRMLLAQKAYCEGALALELYCARLVDEQKTGDAQAADDARLLLEVLTPI
AKSWPSEWCLEANSLAIQVHGGYGYTRDFPVEQYWRDNRLNMIHEGTHGIQAADLLGRKV
LMEKGRGLQLLAGRMTATIASAAKVPALAAHAKALGAALQRIGSATEAAWFTGDPADALA
NAVPYMQAFGHTVLAWIWLDVARRALEKDGEKARAVTAGKIGATDYFFHYELPKIGAWLN
VVETRDPTCTALPEDAF