Protein Info for GFF132 in Sphingobium sp. HT1-2
Annotation: Methylmalonyl-CoA mutase (EC 5.4.99.2) / B12 binding domain of Methylmalonyl-CoA mutase (EC 5.4.99.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 63% identical to SCPA_ECOLI: Methylmalonyl-CoA mutase (scpA) from Escherichia coli (strain K12)
KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 96% identity to sch:Sphch_0762)MetaCyc: 65% identical to methylmalonyl-CoA mutase large subunit (Chloroflexus aurantiacus)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]
Predicted SEED Role
"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)
MetaCyc Pathways
- propanoyl CoA degradation I (3/3 steps found)
- 3-hydroxypropanoate cycle (10/13 steps found)
- 2-oxobutanoate degradation I (3/4 steps found)
- anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
- methylaspartate cycle (13/19 steps found)
- 3-hydroxypropanoate/4-hydroxybutanate cycle (12/18 steps found)
- superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
- pyruvate fermentation to propanoate I (4/7 steps found)
- conversion of succinate to propanoate (1/3 steps found)
- (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
- superpathway of L-methionine salvage and degradation (8/16 steps found)
- L-glutamate degradation VIII (to propanoate) (4/11 steps found)
- crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (4/14 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 5.4.99.2
Use Curated BLAST to search for 5.4.99.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (717 amino acids)
>GFF132 Methylmalonyl-CoA mutase (EC 5.4.99.2) / B12 binding domain of Methylmalonyl-CoA mutase (EC 5.4.99.2) (Sphingobium sp. HT1-2) MTEKPTLDQWAAAAAKEVKGKDLNWDTPEGIVVKPLYTAEDVTVDPGLPGFAPFTRGVRA SMYAGRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVVGDV GKAGVAIDTIEDMKILFDGIPLDKMSVSMTMNGAVIPILAFFIVAGEEQGVERKLLDGTI QNDILKEFMVRNTYIYPPEPSMRIISDIFGYTSREMPKFNSISISGYHMQEAGATQVQEL AFTIADGAEYVRYGVASGLDIDKFAGRLSFFFAIGMNFFMEIAKLRAARVLWHRVMTKLG AQDERSKMLRTHCQTSGVSLTEQDPYNNVMRTTIEAMAAMLGGTQSLHTNALDEAIALPT DFSARIARNTQIVIQEETGMCNVVDPLGGSYYIEALTQQLVDAAQEIIDRVEAEGGMAKA VGAGWPKAMIETAAAARQARVDRGEDVIVGVNKYRLANEDLLETLEVDNTKVREAQIARI NRVKAQRDEDACQTALQSLREAAAAPQSIETNLLAHAVECARARATLGEISAAMEDSFNR YGTQPTPVKGVYGAPYKDDSRWKQVLDGVQAVERRLGRKPKLLVAKMGQDGHDRGANIIA SAFGDMGFDVVSGPLFQTPEETVVLALDSGVDVVGASSLAAGHKTLIPELINRLRDHGRS DIKVIAGGVIPPQDYDYLRDAGVQGIYGPGSNVVECAADVLRLLGHNMPPAGLEEAA