Protein Info for GFF1315 in Xanthobacter sp. DMC5

Annotation: Potassium-transporting ATPase ATP-binding subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 867 transmembrane" amino acids 79 to 99 (21 residues), see Phobius details amino acids 105 to 122 (18 residues), see Phobius details amino acids 253 to 273 (21 residues), see Phobius details amino acids 291 to 322 (32 residues), see Phobius details amino acids 652 to 673 (22 residues), see Phobius details amino acids 679 to 699 (21 residues), see Phobius details amino acids 719 to 742 (24 residues), see Phobius details amino acids 761 to 779 (19 residues), see Phobius details amino acids 791 to 813 (23 residues), see Phobius details amino acids 819 to 844 (26 residues), see Phobius details PF00690: Cation_ATPase_N" amino acids 40 to 97 (58 residues), 47.4 bits, see alignment 1.8e-16 TIGR01647: plasma-membrane proton-efflux P-type ATPase" amino acids 49 to 810 (762 residues), 901.1 bits, see alignment E=5.5e-275 TIGR01494: HAD ATPase, P-type, family IC" amino acids 109 to 533 (425 residues), 171.9 bits, see alignment E=1.8e-54 amino acids 560 to 681 (122 residues), 99.7 bits, see alignment E=1.4e-32 PF00122: E1-E2_ATPase" amino acids 141 to 325 (185 residues), 140 bits, see alignment E=9.4e-45 PF00702: Hydrolase" amino acids 342 to 606 (265 residues), 65.9 bits, see alignment E=1e-21

Best Hits

KEGG orthology group: None (inferred from 84% identity to azc:AZC_0191)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (867 amino acids)

>GFF1315 Potassium-transporting ATPase ATP-binding subunit (Xanthobacter sp. DMC5)
MAQSAARIAAPAPAEAPAAKPAASSGGPVRLDAAAISAKFTELGSSPKGLSATEAAERQA
KYGPNAIVAHEDSRWEKLVGYFWGPIPWMIEAAALLSLIRQDWPDFIVVMGLLIYNAIVG
FWQDSKAASALAALKKGLALKARVLRDNKWMTVDTAALVPGDVVSVAGGETLPADILLVE
GKYLSVDQAALTGESLPVSKGVGDSGYSGSIVRQGTMTGLVTATGNATFFGRTAKLVASA
GAKSHAEKAVIQMGDFLIILSAALALVLVVAQVHRDIIADGHWEWQHAGAIVQIVLVLLV
ASVPVATPAVMSVTMALGALALSKQQAIVSRLSAIEELAGVDVLCSDKTGTLTLNQLTLQ
TPIPWGKAAPEDLILGAALASQRQSDDAIDKAVIAAVKDPKVLDGYKSVDFTPFDPVSKK
TVASVAGPDGKTVHFAKGAPQAIAAQCGLSEADAKGYFDAVEKLARSGTRALGVARSDDG
KSWTLLGLLPMLDPPRPDAAETIAKAKALGVSVKMVTGDDVAIGGEISRQLGLGDHLLVA
SDVFGQDSGPEHIAIDAARAVEVADGFGRVFPEHKFQIVKALQERGHIVAMTGDGVNDAP
ALKQADCGVAVSGATDAARSAAALILTAPGLSTIINAIMEARAIFERITSYIYYRIAMTL
NIMLVVVLVYLVYDFMPLTAIMIVVMALLDDIPIMTIAYDNVKVQDSPVRWNMQRILSFS
TIMGIMALVQSFGIVMLGMFWMKSPELMARLPMDLSHIQTMLFLQLCAGGHLLFFVSRVQ
GTLFRPPYPSLPVLAAVMGTQVFAIFMCAFGWFMPALPWLLIGVVWAYCLVWTLVMDLVK
VIYFRFVNARERRQSMIDASIGPVVPG