Protein Info for GFF1309 in Xanthobacter sp. DMC5

Annotation: Carbon monoxide dehydrogenase medium chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 286 PF00941: FAD_binding_5" amino acids 5 to 174 (170 residues), 160.6 bits, see alignment E=3.3e-51 PF03450: CO_deh_flav_C" amino acids 182 to 281 (100 residues), 82 bits, see alignment E=3.1e-27

Best Hits

Swiss-Prot: 35% identical to DCMM_HYDPS: Carbon monoxide dehydrogenase medium chain (cutM) from Hydrogenophaga pseudoflava

KEGG orthology group: K03519, carbon-monoxide dehydrogenase medium subunit [EC: 1.2.99.2] (inferred from 64% identity to bra:BRADO4586)

MetaCyc: 35% identical to carbon monoxide dehydrogenase medium chain (Afipia carboxidovorans)

Predicted SEED Role

"Carbon monoxide dehydrogenase medium chain (EC 1.2.99.2)" in subsystem CO Dehydrogenase (EC 1.2.99.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.99.2

Use Curated BLAST to search for 1.2.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (286 amino acids)

>GFF1309 Carbon monoxide dehydrogenase medium chain (Xanthobacter sp. DMC5)
LKARAFAYARPSSLAEAHAAFAGADGDASYISGGQSLVPALAMRLQAPQVLIDLSAIPEM
RGVLLAGENLRIGALTRHAEALTEPLIRAYAPLLAAAAPFVAHPAIRNRGTVGGSVALAD
PASEFPAMMLAMGAEMEISGADGIRRVAADDYFQDLYQTAIEPGEILVALHVPAAGPRHV
FAFDEMARRRGDYAMVGGGILAERDGAVIANPRIALFSVGSTPVRARNAEAEIAGKALDA
DTIAAAQRALDADIDPLDDAEIPAAMRRHLARVLLGRLLRKLETCS