Protein Info for GFF1295 in Sphingobium sp. HT1-2

Annotation: Isoleucyl-tRNA synthetase (EC 6.1.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 961 TIGR00392: isoleucine--tRNA ligase" amino acids 18 to 897 (880 residues), 795 bits, see alignment E=4.9e-243 PF00133: tRNA-synt_1" amino acids 32 to 691 (660 residues), 429.1 bits, see alignment E=2.5e-132 PF09334: tRNA-synt_1g" amino acids 61 to 206 (146 residues), 39.4 bits, see alignment E=5e-14 PF08264: Anticodon_1" amino acids 747 to 895 (149 residues), 78.2 bits, see alignment E=9.4e-26

Best Hits

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (961 amino acids)

>GFF1295 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Sphingobium sp. HT1-2)
MTDQPDYKSTVFLPVTDFPMKAGLAQKEPAIAARWAAMDLYGKLREKRAGRERFILHDGP
PYANGDIHMGHAMNKVLKDIIVRSQSLLGKDAPYVPGWDCHGLPIEWKIEEEYRKKKMNK
DEVPASEFRAQCRAYADKWVDVQKEQFKRLGVMGDWADPYLTMKFDAEATIVGELLKFAE
SGQLYRGAKPVMWSPVEKTALAEAEVEYEDITSTQIDVAFEIVEAPNAPELVGAHAVIWT
TTPWTIPVNQALAYGPEVDYAITSCKDHDGKLLGRFLIATELVAAFQARADAAVRGVENP
ELVGAWAIEGFVNGSDLAGAVARHPMHALGGFFAKPRPFLAGDFVTTDAGTGLVHMAPDH
GEDDFALCKANGLNPVFAVEGDGKYRADWLWLGGQGSVINPKFVGKDGPICSDLREAGAL
LAASDDFKHSYPHSWRSKAKIIFRCTPQWFIPMDKPQEGVVADVDGGVMPTPIVTGNGPT
LREIALDAIEHTRWVPERSTNRIRSMVEGRPDWVISRQRAWGVPIALYVHRKTGDYLVDK
AVNARIIEAFKGAGADAWFGADHQALLGPDYDLADYEVVNDILDVWFDSGSTHSFVVEGR
YGEGTRADLYIEGSDQHRGWFQSSLLESCGTRGQAPYKAVLTHGFALDGSGKKMSKSLGN
VVDPLKIMAESGADILRVWVASTDYFDDVRIGKEVLAGSSDAYRKLRNTFRYMLGALSDY
DESEAVSYAEMPELERYMLHRLAQLDAELRAVVDKAAKSENWLEFSRYTRAIFDFANSDL
SAFFFDIRKDCLYCDAKSDPKRRAYRTLLDTLFHALVRYVAPIIPFTAEEVWQSRFPSDE
DSVHFLEWPEVDHRWIDSHLNDKWAELRDQREQVNEAIEPFRREKVIRSSLEADVTMGEM
LPSDGVNFAEIAIVARIEMGVGDGIIVKPSEWHKCGRCWRLLPEVEEDGTLCNRCDEVLK
A