Protein Info for GFF1289 in Sphingobium sp. HT1-2

Annotation: 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13292: DXP_synthase_N
  • amino acids 9 to 282 (274 residues), 388.7 bits, E=2.8e-120
• TIGR00204: 1-deoxy-D-xylulose-5-phosphate synthase
  • amino acids 12 to 627 (616 residues), 787.1 bits, E=6.2e-241
• PF00676: E1_dh
  • amino acids 117 to 183 (67 residues), 24.5 bits, E=2.6e-09
• PF02779: Transket_pyr
  • amino acids 319 to 480 (162 residues), 156.9 bits, E=8.5e-50
• PF02780: Transketolase_C
  • amino acids 496 to 619 (124 residues), 94.1 bits, E=1.3e-30

Best Hits

Swiss-Prot: 83% identical to DXS_SPHAL: 1-deoxy-D-xylulose-5-phosphate synthase (dxs) from Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)

KEGG orthology group: K01662, 1-deoxy-D-xylulose-5-phosphate synthase [EC: 2.2.1.7] (inferred from 91% identity to sjp:SJA_C1-22860)

MetaCyc: 56% identical to 1-deoxy-D-xylulose 5-phosphate synthase (Arabidopsis thaliana col)
1-deoxy-D-xylulose-5-phosphate synthase. [EC: 2.2.1.7]

Predicted SEED Role

"1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)" in subsystem Isoprenoid Biosynthesis or Pyridoxin (Vitamin B6) Biosynthesis or Thiamin biosynthesis (EC 2.2.1.7)

MetaCyc Pathways

• superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) (12/12 steps found)
• taxadiene biosynthesis (engineered) (12/13 steps found)
• methylerythritol phosphate pathway I (9/9 steps found)
• methylerythritol phosphate pathway II (9/9 steps found)
• isoprene biosynthesis I (9/10 steps found)
• pyridoxal 5'-phosphate biosynthesis I (6/7 steps found)
• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (8/12 steps found)
• superpathway of thiamine diphosphate biosynthesis II (7/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (6/10 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (3/7 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (2/6 steps found)
• superpathway of ergosterol biosynthesis II (11/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Terpenoid biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.2.1.7

Protein Sequence (639 amino acids)

>GFF1289 1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7) (Sphingobium sp. HT1-2)
MSNPSTPLLDTVKIPADLRKLQPDQLRQLADELRAETIAAVGVTGGHLGSGLGVVELTTA
IHYVFDTPNDKLVWDVGHQCYPHKILTGRRDRIRTLRQGGGLSGFTKRAESEYDPFGAAH
SSTSISAALGFAVANKMQDKPGKGIAVIGDGAMSAGMAYEAMNNAREAGNRLVVILNDND
MSIAPPVGGLSAYLARLVSSREFLGLRDMAKKLARKLPRPLHKAAKKTDEFARGMATGGT
LFEELGFYYVGPIDGHNLEHLIPVLENVRDAAEGPCLIHVVTQKGKGYGPAEAAADKYHG
VQKFDVITGTQTKAPPGPPSYTNVFAQALIAEAQRDPRVCAITAAMPSGTGLDKFELAFP
DRTFDVGIAEQHAVTFAAGLAAEGMRPFCAIYSTFLQRAYDQVVHDVAIQNLPVRFAIDR
AGLVGADGSTHAGSFDVTYLASLPNFVVMAAADEAELTHMVHTCAVHDSGPIAVRYPRGN
GTGVAMPEVPEALEIGKGRVVREGRQVAILSLGTRLEEAMKAADTLEAKGLSASVVDLRF
AKPLDEALIRRMLTTHEVAVTIEEGSIGGLGAHVLTLASDLGLTDNGLKIRTMRLPDIFQ
DQDKPEQQYADARLDADAIVDTVLTALRHNSAGVEEARA